Key features and details
- Mouse monoclonal [MY-32] to Fast Myosin Skeletal Heavy chain
- Suitable for: ICC, IHC-P
- Reacts with: Rabbit
- Isotype: IgG1
产品名称Anti-Fast Myosin Skeletal Heavy chain抗体[MY-32]
参阅全部 Fast Myosin Skeletal Heavy chain 一抗
描述小鼠单克隆抗体[MY-32] to Fast Myosin Skeletal Heavy chain
经测试应用适用于: ICC, IHC-Pmore details
预测可用于: Mouse, Rat, Chicken, Guinea pig, Cow
Full length native protein (purified) corresponding to Rabbit Fast Myosin Skeletal Heavy chain. Purified rabbit muscle myosin.
- ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612). IHC: Rabbit tongue section
This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lot is from ascites and is still in stock as of 18th October 2016 (GR255796). Lot numbers higher than GR255796 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
May be used for detecting cross striated muscle differentiation in tumors.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.097% Sodium azide
Concentration information loading...
纯化说明Purified from Tissue culture supernatant.
序列相似性Contains 1 IQ domain.
Contains 1 myosin head-like domain.
结构域The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
细胞定位Cytoplasm > myofibril. Thick filaments of the myofibrils.
- Information by UniProt
- adult 1 antibody
- MYH1 antibody
- MYH1_HUMAN antibody
Immunofluorescence staining of Fast Myosin Skeletal Heavy chain using ab51263 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 3 (left panel), 5 (middle panel) and 10 days (right panel) post induction.
The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab51263 at 5 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab51263 gave comparable results using 4% formaldehyde fixation (10 min).
Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Rabbit tongue sections labeling Fast Myosin Skeletal Heavy chain with ab51263 at 20 μg/mL followed by Goat Anti-Mouse IgG (Fab specific)-FITC antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51263 被引用在 66 文献中.
- Cefis M et al. Endothelial cells are an important source of BDNF in rat skeletal muscle. Sci Rep 12:311 (2022). PubMed: 35013359
- Chi MY et al. Silibinin Alleviates Muscle Atrophy Caused by Oxidative Stress Induced by Cisplatin through ERK/FoxO and JNK/FoxO Pathways. Oxid Med Cell Longev 2022:5694223 (2022). PubMed: 35096269
- Li S et al. Endoplasmic Reticulum Stress Contributes to Ventilator-Induced Diaphragm Atrophy and Weakness in Rats. Front Physiol 13:897559 (2022). PubMed: 35832486
- Greene MA et al. Impact of fetal exposure to mycotoxins on longissimus muscle fiber hypertrophy and miRNA profile. BMC Genomics 23:595 (2022). PubMed: 35971074
- Sun M et al. Research Note: Creatine monohydrate alleviates protein breakdown induced by corticosterone via inhibiting ubiquitin proteasome pathway in chicken myotubes. Poult Sci 101:102177 (2022). PubMed: 36194918