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Anti-Fast Myosin Skeletal Heavy chain抗体[MY-32] (ab51263)

Reviews (6)Q&A (7)References (66)
Immunocytochemistry - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)

Key features and details

  • Mouse monoclonal [MY-32] to Fast Myosin Skeletal Heavy chain
  • Suitable for: ICC, IHC-P
  • Reacts with: Rabbit
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Fast Myosin Skeletal Heavy chain抗体[MY-32]
    参阅全部 Fast Myosin Skeletal Heavy chain 一抗

  • 描述

    小鼠单克隆抗体[MY-32] to Fast Myosin Skeletal Heavy chain

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC, IHC-Pmore details

  • 种属反应性

    与反应: Rabbit
    预测可用于: Mouse, Rat, Chicken, Guinea pig, Cow

  • 免疫原

    Full length native protein (purified) corresponding to Rabbit Fast Myosin Skeletal Heavy chain. Purified rabbit muscle myosin.

  • 阳性对照

    • ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612). IHC: Rabbit tongue section

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lot is from ascites and is still in stock as of 18th October 2016 (GR255796). Lot numbers higher than GR255796 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    May be used for detecting cross striated muscle differentiation in tumors.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab51263于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use a concentration of 1 - 5 µg/ml.
IHC-P (4)
Use at an assay dependent concentration.
说明
ICC
Use a concentration of 1 - 5 µg/ml.
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Muscle contraction.

  • 序列相似性

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.

  • 结构域

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

  • 细胞定位

    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt

  • 数据库链接

    • 别名

      • adult 1 antibody
      • MYH1 antibody
      • MYH1_HUMAN antibody
      • MyHC-2x antibody
      • MyHC-IIx/d antibody
      • Myosin heavy chain 1 antibody
      • Myosin heavy chain 2x antibody
      • Myosin heavy chain antibody
      • Myosin heavy chain IIx/d antibody
      • Myosin-1 antibody
      • skeletal muscle antibody
      see all

    图片

    • Immunocytochemistry - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)
      Immunocytochemistry - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)

      Immunofluorescence staining of Fast Myosin Skeletal Heavy chain using ab51263 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 3 (left panel), 5 (middle panel) and 10 days (right panel) post induction.


      The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab51263 at 5 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).


      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.


      The antibody ab51263 gave comparable results using 4% formaldehyde fixation (10 min).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)
      Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Rabbit tongue sections labeling Fast Myosin Skeletal Heavy chain with ab51263 at 20 μg/mL followed by Goat Anti-Mouse IgG (Fab specific)-FITC antibody.
       

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (66)

    发表研究结果有使用 ab51263？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab51263 被引用在 66 文献中.

    • Cefis M  et al. Endothelial cells are an important source of BDNF in rat skeletal muscle. Sci Rep 12:311 (2022). PubMed: 35013359
    • Chi MY  et al. Silibinin Alleviates Muscle Atrophy Caused by Oxidative Stress Induced by Cisplatin through ERK/FoxO and JNK/FoxO Pathways. Oxid Med Cell Longev 2022:5694223 (2022). PubMed: 35096269
    • Li S  et al. Endoplasmic Reticulum Stress Contributes to Ventilator-Induced Diaphragm Atrophy and Weakness in Rats. Front Physiol 13:897559 (2022). PubMed: 35832486
    • Greene MA  et al. Impact of fetal exposure to mycotoxins on longissimus muscle fiber hypertrophy and miRNA profile. BMC Genomics 23:595 (2022). PubMed: 35971074
    • Sun M  et al. Research Note: Creatine monohydrate alleviates protein breakdown induced by corticosterone via inhibiting ubiquitin proteasome pathway in chicken myotubes. Poult Sci 101:102177 (2022). PubMed: 36194918
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 13 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Anal Sphincter Skeletal Muscle)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6
    Permeabilization
    No
    Specification
    Anal Sphincter Skeletal Muscle
    Blocking step
    BLOXALL first then Leica Superblock each as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Hannah Ruetten

    Verified customer

    提交于 Aug 18 2023

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cow Tissue sections (Muscle)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Antigen Retrieval Buffer ph 9.0
    Permeabilization
    Yes - 0.1% Tween
    Specification
    Muscle
    Blocking step
    10% goat serum in 0.1% Tween/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. Rhonda Powell

    Verified customer

    提交于 Mar 08 2022

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Spalax (blind mole rat) Cell (muscle tissue (Paraffin sections))
    Permeabilization
    No
    Specification
    muscle tissue (Paraffin sections)
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Lital Sharvit

    Verified customer

    提交于 Jun 07 2021

    Western blot abreview for Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Skeletal muscle cells)
    Gel Running Conditions
    Reduced Denaturing (8% acrylamide)
    Loading amount
    30 µg
    Specification
    Skeletal muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 11 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Skeletal muscle)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Skeletal muscle
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    提交于 Aug 21 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Skeletal muscle)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Skeletal muscle
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    提交于 Aug 20 2018

    Question

    We purchased the primary antibody against fast myosin skeletal heavy chain (My-32-ab51263) and would like to confirm against which fast myosin isoform it is specific. Does it stain all myo fast isoforms (as seems to be stated in the product overview)?

    Read More
    Answer

    I can confirm that ab51263 Fast Myosin Skeletal Heavy chain antibody [MY-32] was produced from spleen of mouse immunised with rabbit muscle myosin. I am sorry that regrettably no other specification as to its epitope identity has been assessed. Nevertheless, this antibody has been used in many papers (a few of them are cited below), which I hope may be useful to you:


    PNAS 86; 7007-7011 (1989)

    Development 107; 751-769 (1989)

    Histochemistry 93; 497-499 (1990)

    Histopathology 17; 301-309 (1990)

    Differentiation 55; 203-211 (1994)

    I am sorry we do not have the specific information you require on this occasion, but I hope this will be helpful.

    Read More

    Question

    rofter I contacted with this customer, she indicated she would like to change a free of replacement with ab7784 (Anti-fast skeletal Myosin antibody [MY-32]),

    could you please help this customer to solve the problem?

    Thanks for your kindly help

    Read More
    Answer

    r these details as this enables us to closely monitor the quality of our products

    We would be pleased to arrange a replacement product free of charge in order number#

    Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

    Read More

    Question

    Product code: 51263

    Inquiry: 1) Order details: ˙Batch number (Lot number): gr25963-2 Po: 1069725 ˙Abcam product code: ab51263 ˙Antibody storage conditions (temperature/reconstitution etc) : -20℃
    2) Please describe the problem (high background, non-specific signal…etc). It can work with ab11083, but non-specific signal with ab51263 (same condition and same muscle).
    3) On what material are you testing the antibody in IHC/ICC? ˙Species: mice (C57/BL6) ˙What’s cell line or tissue: skeletal muscle
    4) Sample preparation: ˙Type of sample: formalin fixed ˙paraffin embedded sections, cells in culture, other:____) : paraffin embedded sections ˙Sample preparation: ˙Positive control : ˙Negative control :
    5) Fixation step ˙Yes or No : Yes ˙If yes, Fixative agent and concentration: 10 % neutral buffer formalin ˙Fixation time: ˙Fixation temperature: room temperature
    6) Antigen retrieval method(including time, temperature etc.): heat 15 mins
    7) Permeabilization method: ˙Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers? ˙Permeabilizing agent and concentration:
    8) Blocking agent (eg BSA, serum…): ˙Concentration: 3% ˙Blocking time :2 hr ˙Blocking temperature: room temperature
    9) ˙Endogenous peroxidases blocked? ˙Endogenous biotins blocked?
    10) Primary antibody (If more than one was used, describe in “additional notes”) : ˙Species: mice ˙Reacts against: ˙At what dilution(s) have you tested this antibody:1:100 and 1:150 ˙Diluent buffer: 1% BSA ˙Incubation time: overnight ˙Incubation temperature: 4℃ ˙What washing steps were done (which buffer, number of washes): TBST, 3×10 mins
    11) Secondary antibody: supersensitivy polymer-HRP IHC detecting system, QD4200810(Lot number) ˙Species: ˙Reacts against which species: ˙At what dilution(s) have you tested this antibody: ˙Diluent buffer: ˙Incubation time: ˙What washing steps were done (which buffer, number of washes): ˙Fluorochrome or enzyme conjugate (eg: FITC, HRP, AP, biotin…etc): ˙Do you know whether the problems you are experiencing come from the secondary?
    12) Signal amplification method (eg: ABC, LSAB, HRP polymer, TSE): HRP polymer
    13) Detection method (eg: DAB, BCIP/NBT …etc):DAB
    14) ˙ How many times have you run this staining? 3 times ˙Do you obtain the same results every time? YES ˙What steps have you altered to try and optimize the use of this antibody? antibody dilution(s)¸1:150 to 1:100

    Read More
    Answer

    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab51263 Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]. I would also appreciate if you can confirm some further details:

    1. Please keep in mind that you cannot compare two different antibodies against two different targets with each other. Every antibody needs its individual optimization.

    2. What was the HIER buffer? Did the customer perform a time series to find the optimal point? I would strongly recommend to perform a time series of at least 3 different retrieval times (like 15, 20, 25 min) to find the optimal time. As every antibody is different, every epitope may show a different retrieval time. The occurrence ofno signalusually shows a too short retrieval.

    3. Isthe customer using a detergent like Tween or Triton during incubation? If not, I can recommend adding Triton to your incubation solution to facilitate the antibodies solubility, but in a low concentration of 0.025%.

    Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    Read More

    Question

    Could you tell us the results.
    It's been a long time since last question. Thanks

    Read More
    Answer

    Apologies for the delay, I was waiting for further information.

    Our products team has confirmed that these products have the correct alternative names listed. As stated on the datasheets they will specifically stain either the fast or slow fibers.

    I hope this information is useful.

    Read More

    1-10 of 13 Abreviews or Q&A

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