Anti-Fas Ligand抗体(ab134401)
Key features and details
- Rabbit polyclonal to Fas Ligand
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Fas Ligand抗体
参阅全部 Fas Ligand 一抗 -
描述
兔多克隆抗体to Fas Ligand -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human Fas Ligand aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: P48023 -
阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Human Normal prostate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134401于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. -
疾病相关
Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
N-glycosylated.
The soluble form derives from the membrane form by proteolytic processing. -
细胞定位
Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface. - Information by UniProt
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数据库链接
- Entrez Gene: 356 Human
- Entrez Gene: 14103 Mouse
- Omim: 134638 Human
- SwissProt: P48023 Human
- SwissProt: P41047 Mouse
- Unigene: 2007 Human
- Unigene: 3355 Mouse
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别名
- ALPS1B antibody
- Apoptosis (APO 1) antigen ligand 1 antibody
- Apoptosis antigen ligand 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas Ligand antibody (ab134401)
IHC image of Fas Ligand staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134401, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ICC/IF image of ab134401 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab134401 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab134401 被引用在 1 文献中.
- Li GM et al. Heat stress decreases egg production of laying hens by inducing apoptosis of follicular cells via activating the FasL/Fas and TNF-a systems. Poult Sci 99:6084-6093 (2020). PubMed: 33142528