重组Anti-Fas抗体[EPR21088] - BSA and Azide free

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Fas antibody [EPR21088] - BSA and Azide free (AB227907)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse spleen tissue (fresh) labelling Fas with ab216991 at 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary at 1/1000 dilution. Positive staining on mouse spleen tissue is observed. The nuclear counterstain was DAPI (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216991).

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fas antibody [EPR21088] - BSA and Azide free (AB227907)

Flow cytometric analysis of primary mouse thymocytes labeling Fas with ab216991 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Total viable cells were gated for the image.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216991).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fas antibody [EPR21088] - BSA and Azide free (AB227907)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized primary mouse thymocytes labeling Fas with ab216991 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on primary mouse thymocytes.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control : L-929 (PMID : 7521365).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216991).

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fas antibody [EPR21088] - BSA and Azide free (AB227907)

Negative control :  Flow cytometric analysis of L-929 (mouse connective tissue fibroblast cell line) cell line labeling Fas with ab216991 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control : L-929 (PMID : 12077239).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216991).