Anti-FARSLA抗体(ab224049)
Key features and details
- Rabbit polyclonal to FARSLA
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-FARSLA抗体 -
描述
兔多克隆抗体to FARSLA -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Orangutan
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免疫原
Recombinant fragment corresponding to Human FARSLA aa 1-200.
Database link: Q9Y285 -
阳性对照
- IHC: Human duodenum tissue. WB: RT4, U-251 MG, NIH/3T3 and NBT-II cell lysate; human liver and tonsil tissue lysate. ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224049于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 58 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 58 kDa. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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相关性
FARSLA (Phenylalanyl-tRNA synthetase alpha chain) is an aminoacyl-tRNA synthetase. These are a class of enzymes that charge tRNAs with their cognate amino acids. Cytoplasmic phenylalanine-tRNA synthetase is a heterodimer consisting of a catalytic alpha subunit, FARSLA, and a regulatory beta subunit, FARSLB. This protein has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase family shown to exhibit this type of regulated expression. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 2193 Human
- Entrez Gene: 66590 Mouse
- Entrez Gene: 288917 Rat
- Omim: 602918 Human
- SwissProt: Q9Y285 Human
- SwissProt: Q8C0C7 Mouse
- SwissProt: Q5RFA2 Orangutan
- SwissProt: Q505J8 Rat
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别名
- CML 33 antibody
- CML33 antibody
- FARS antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FARSLA antibody (ab224049)
Paraffin-embedded human duodenum tissue stained for FARSLA using ab224049 at 1/1000 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-FARSLA antibody (ab224049)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for FARSLA (green) using ab224049 at 4 µg/ml in ICC/IF.
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Western blot - Anti-FARSLA antibody (ab224049)All lanes : Anti-FARSLA antibody (ab224049) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 58 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224049 尚未被引用在任何文献中。