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重组RabMAb

重组Anti-FANCI抗体[EPR23903-124] - BSA and Azide free (ab273628)

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Immunocytochemistry/ Immunofluorescence - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
  • Flow Cytometry (Intracellular) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
  • Flow Cytometry (Intracellular) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
  • Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23903-124] to FANCI - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-FANCI抗体[EPR23903-124] - BSA and Azide free
    参阅全部 FANCI 一抗

  • 描述

    兔单克隆抗体[EPR23903-124] to FANCI - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
    不适用于: IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, HeLa treated with 500ng/ml Mitomycin C for 6h, whole, Jurkat and Raji whole cell lysates; Human testis tissue lysate. IHC-P: Human testis, colon and tonsil tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa and Jurkat cells.

  • 常规说明

    ab273628 is the carrier-free version of ab245219.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273628于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.

  • 疾病相关

    Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.

  • 结构域

    The C-terminal 30 residues are probably required for function in DNA repair.

  • 翻译后修饰

    Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
    Phosphorylated in response to DNA damage by ATM and/or ATR.

  • 细胞定位

    Nucleus. Concentrates in nuclear foci upon genotoxic stress.
  • Information by UniProt

  • 数据库链接

    • 别名

      • FANCI antibody
      • FANCI gene antibody
      • FANCI_HUMAN antibody
      • Fanconi anemia group I protein antibody
      • Fanconi anemia, complementation group I antibody
      • FLJ10719 antibody
      • FLJ14658 antibody
      • KIAA1794 antibody
      • Protein FACI antibody
      • Protein FANCI antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
      Immunocytochemistry/ Immunofluorescence - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)

      Immunofluorescent analysis of 100% methanol-fixed HeLa cells labelling FANCI with ab245219 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear foci staining in HeLa cell line, the signal increased a little after treatment with Mitomycin C (500ng/ml) for 6h. FANCI forms nuclear foci in cells in the absence or presence of exogenous DNA damage (PMID: 19561358). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245219).

    • Flow Cytometry (Intracellular) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
      Flow Cytometry (Intracellular) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling FANCI with ab245219 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

      A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245219). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FANCI with ab245219 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human tonsil. The section was incubated with ab245219 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245219).

    • Flow Cytometry (Intracellular) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
      Flow Cytometry (Intracellular) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling FANCI with ab245219 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

      A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245219). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FANCI with ab245219 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human colon. The section was incubated with ab245219 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245219).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling FANCI with ab245219 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human testis (PMID: 31219578). The section was incubated with ab245219 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245219).

    • Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)
      Anti-FANCI antibody [EPR23903-124] - BSA and Azide free (ab273628)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab273628？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273628 尚未被引用在任何文献中。

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