AB15344

Anti-FANCI 抗体

Name: FANCI抗体 (ab15344)| Abcam中文官网
Brand: Abcam Limited
SKU: AB15344
Price: 4910 CNY
Availability: InStock
Rating: 4 (6 reviews)

Anti-FANCI antibody

(6 Reviews)

(9 Publications)

Rabbit Polyclonal FANCI antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human FANCI aa 200-250.

查看别名

KIAA1794, FANCI, Fanconi anemia group I protein, Protein FACI

1 Images

Supplier Data

Western blot - Anti-FANCI antibody (AB15344)

All lanes:

Western blot - Anti-FANCI antibody (ab15344) at 1/5000 dilution

Lane 1:

HeLa nuclear extract at 5 µg

Lane 2:

HeLa nuclear extract at 20 µg

Predicted band size: 149 kDa

true

Exposure time: 15min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human FANCI aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

Q9NVI1

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

FANCI also known as Fanconi anemia group I protein is an important player in DNA repair processes. This protein has a molecular mass of approximately 150 kDa. It is ubiquitously expressed with notable levels found in tissues that undergo high rates of cell division. FANCI participates mechanically within the DNA repair mechanism by interacting directly with DNA alongside its partner protein FANCD2 to form a tight complex that recognizes and binds to sites of DNA damage.

Biological function summary

The FANCI protein acts as part of the multiprotein Fanconi anemia (FA) core complex. This complex Functions in the repair of DNA interstrand crosslinks which are severe forms of DNA damage. Successful function of FANCI is important for maintaining genomic stability and subsequently proper cell cycle progression. It partners with proteins including FANCD2 to coordinate the homologous recombination repair process. Defective FANCI leads to disrupted DNA repair resulting in cellular damage and apoptosis.

Pathways

FANCI plays a major role in the Fanconi anemia pathway and the broader DNA damage response system. The FA pathway which involves over 20 proteins forms a network integral in repairing DNA crosslinks. FANCI tightly interacts with proteins like FANCD2 to initiate DNA repair in response to damage. The successful coordination of these proteins ensures the stability of genetic material throughout the cell cycle affecting cell fate decisions such as survival or apoptosis.

FANCI mutations are directly linked to Fanconi anemia a genetic disorder characterized by bone marrow failure and increased cancer risk. Mutations can impair the protein’s function causing defective DNA repair capability. Additionally FANCI's involvement in DNA repair associates it with breast cancer where malfunction or reduced expression of FANCI is observed. The protein interacts with tumor suppressors like BRCA1 and BRCA2 linking it to pathways involved in hereditary breast cancer predisposition.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA : binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.

See full target information FANCI

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 41:552-558 PubMed30365131

2018

BRMS1 participates in regulating cell sensitivity to DNA interstrand crosslink damage by interacting with FANCI.

Applications

Unspecified application

Species

Unspecified reactive species

Jianming Dou,Yiren Zhou,Xuni Liu,Xiaojing Qiao,Xi Yang,Wenjuan Xie,Shouyi Qiao,Yanhua Wu

Cancer cell 34:315-330.e7 PubMed30033091

2018

MAST1 Drives Cisplatin Resistance in Human Cancers by Rewiring cRaf-Independent MEK Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Lingtao Jin,Jaemoo Chun,Chaoyun Pan,Dan Li,Ruiting Lin,Gina N Alesi,Xu Wang,Hee-Bum Kang,Lina Song,Dongsheng Wang,Guojing Zhang,Jun Fan,Titus J Boggon,Lu Zhou,Jeanne Kowalski,Cheng-Kui Qu,Conor E Steuer,Georgia Z Chen,Nabil F Saba,Lawrence H Boise,Taofeek K Owonikoko,Fadlo R Khuri,Kelly R Magliocca,Dong M Shin,Sagar Lonial,Sumin Kang

Cell 169:1105-1118.e15 PubMed28575672

2017

A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability.

Applications

Unspecified application

Species

Unspecified reactive species

Shawn Lu Wen Tan,Saakshi Chadha,Yansheng Liu,Evelina Gabasova,David Perera,Karim Ahmed,Stephanie Constantinou,Xavier Renaudin,MiYoung Lee,Ruedi Aebersold,Ashok R Venkitaraman

Spermatogenesis 5:e979061 PubMed26413409

2015

Uncoupling of transcription and translation of Fanconi anemia (FANC) complex proteins during spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Duangporn Jamsai,Anne E O'Connor,Liza O'Donnell,Jennifer Chi Yi Lo,Moira K O'Bryan

The Journal of clinical investigation 123:3839-47 PubMed23934222

2013

Fanconi anemia signaling network regulates the spindle assembly checkpoint.

Applications

Species

Human

Grzegorz Nalepa,Rikki Enzor,Zejin Sun,Christophe Marchal,Su-Jung Park,Yanzhu Yang,Laura Tedeschi,Stephanie Kelich,Helmut Hanenberg,D Wade Clapp

Cell reports 4:589-600 PubMed23911284

2013

Locus-specific proteomics by TChP: targeted chromatin purification.

Applications

Species

Human

Farzin Pourfarzad,Ali Aghajanirefah,Ernie de Boer,Sara Ten Have,Thamar Bryn van Dijk,Sima Kheradmandkia,Ralph Stadhouders,Supat Thongjuea,Eric Soler,Nynke Gillemans,Marieke von Lindern,Jeroen Demmers,Sjaak Philipsen,Frank Grosveld

Cell cycle (Georgetown, Tex.) 10:3300-10 PubMed21926477

2011

The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms.

Applications

Species

Human

Wei-Ting Lu,Kimon Lemonidis,Ross M Drayton,Thierry Nouspikel

Human molecular genetics 20:2549-59 PubMed21478198

2011

Snm1B/Apollo functions in the Fanconi anemia pathway in response to DNA interstrand crosslinks.

Applications

Species