Anti-FAN1抗体(ab95171)
Key features and details
- Rabbit polyclonal to FAN1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FAN1抗体
参阅全部 FAN1 一抗 -
描述
兔多克隆抗体to FAN1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region within amino acids 1-50 of Human MTMR15, NP_055782.3
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阳性对照
- HeLa cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95171于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 114 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template. -
序列相似性
Belongs to the FAN1 family.
Contains 1 UBZ-type zinc finger.
Contains 1 VRR-NUC domain. -
结构域
The UBZ-type zinc finger specifically binds monoubiquitinated FANCD2. -
细胞定位
Nucleus. Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2. - Information by UniProt
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数据库链接
- Entrez Gene: 22909 Human
- Omim: 613534 Human
- SwissProt: Q9Y2M0 Human
- Unigene: 584863 Human
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别名
- 6030441H18Rik antibody
- Coiled coil domain containing protein MTMR15 antibody
- DKFZp451H236 antibody
see all
图片
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Western blot - Anti-FAN1 antibody (ab95171)All lanes : Anti-FAN1 antibody (ab95171) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 114 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAN1 antibody (ab95171)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling FAN1 with ab95171 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-FAN1 antibody (ab95171)3µg ab95171 were used to immunoprecipitate MTMR15 from 1mg HeLa whole cell lysate.
20% IP was loaded and probed with ab95171 at 1µg/ml.
Detection: chemiluminescence with exposure time of 10 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab95171 被引用在 2 文献中.
- Lim SW et al. Modeling of FAN1-Deficient Kidney Disease Using a Human Induced Pluripotent Stem Cell-Derived Kidney Organoid System. Cells 12:N/A (2023). PubMed: 37759541
- Kim KH et al. Genetic and Functional Analyses Point to FAN1 as the Source of Multiple Huntington Disease Modifier Effects. Am J Hum Genet 107:96-110 (2020). PubMed: 32589923