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Anti-FAM91A1抗体(ab81618)

Reviews (3) Submit a question References (1)
Immunoprecipitation - Anti-FAM91A1 antibody (ab81618)

    Key features and details

    • Rabbit polyclonal to FAM91A1
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-FAM91A1抗体

    • 描述

      兔多克隆抗体to FAM91A1

    • 宿主

      Rabbit

    • 经测试应用

      适用于: IPmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus

    • 免疫原

      Synthetic peptide within Human FAM91A1 aa 300-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_659400.2
      Database link: Q658Y4

    • 阳性对照

      • Whole cell lysate from HeLa cells.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • 存储溶液

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 纯化说明

      ab81618 was affinity purified using an epitope specific to FAM91A1 immobilized on solid support.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab81618于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IP
    说明
    应用说明
    IP: Use at 2 - 5 µg/mg of lysate.

    Is unsuitable for WB.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    靶标

    图片

    • Immunoprecipitation - Anti-FAM91A1 antibody (ab81618)
      Immunoprecipitation - Anti-FAM91A1 antibody (ab81618)
      Detection of FAM91A1 by Western Blot of immunoprecipitates in whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. ab81618 was used for IP at 3 µg/mg lysate (lane 1). Lane 2 represents control IgG. Detection was by chemiluminescence with an exposure time of 3 seconds.

    文献 (1)

    发表研究结果有使用 ab81618？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab81618 被引用在 1 文献中.

    • Navarro Negredo P  et al. The WDR11 complex facilitates the tethering of AP-1-derived vesicles. Nat Commun 9:596 (2018). PubMed: 29426865

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-FAM91A1 antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - other (Hek293T)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Hek293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 31 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FAM91A1 antibody

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - Tx100
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 28 2020

    Western blot abreview for Anti-FAM91A1 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hap1 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-Glycine)
    Loading amount
    5 µg
    Specification
    Hap1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 18 2019

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