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AB249908

重组Anti-FAM83H抗体[EPR12542] - BSA and Azide free

Anti-FAM83H antibody [EPR12542] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal FAM83H antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

Protein FAM83H, FAM83H

1 Images
Western blot - Anti-FAM83H antibody [EPR12542] - BSA and Azide free (AB249908)
  • WB

Supplier Data

Western blot - Anti-FAM83H antibody [EPR12542] - BSA and Azide free (AB249908)

This data was developed using ab176329, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FAM83H antibody [EPR12542] (<a href='/products/primary-antibodies/fam83h-antibody-epr12542-ab176329'>ab176329</a>) at 1/1000 dilution

Lane 1:

HT-1376 lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Lane 3:

HeLa lysate at 10 µg

Predicted band size: 127 kDa,73 kDa

false

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12542

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

ab249908 is the carrier-free version of ab176329.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

FAM83H also known as Family with Sequence Similarity 83 Member H is a protein with a mass of approximately 127 kDa. This protein is found mainly in dental tissues including ameloblasts where it plays a role in enamel formation. Its expression can also be detected in other tissues indicating its importance beyond enamel mineralization. FAM83H-localized at the cell membrane involves interactions with other proteins contributing to cellular processes.
Biological function summary

FAM83H acts as an important player in the mineralization of enamel. It may not seem like it but during enamel development FAM83H ensures proper structural integrity and stability of the enamel matrix. FAM83H is not a part of any complex but associates with other proteins such as casein kinase 1 which phosphorylates it regulating its activity in the amelogenesis process. By maintaining normal enamel development FAM83H aids in dental health and disease prevention.

Pathways

FAM83H functions in processes related to enamel formation and dental tissue development. This protein associates with the Wnt signaling pathway important for regulating cell proliferation and differentiation in various tissues. FAM83H interacts with proteins like Axin an important component of the Wnt pathway modulating cell signaling for proper tooth development. Wnt pathway's influence is detectable in many biological processes confirming FAM83H's role in maintaining essential signaling networks within cells.

FAM83H mutations link to amelogenesis imperfecta a disorder characterized by defective enamel formation. This condition can lead to issues such as abnormal tooth appearance and increased susceptibility to dental caries. FAM83H mutations alter gene functionality impacting its interaction with proteins like casein kinase 1. Another disease associated with FAM83H is Ehlers-Danlos syndrome though this connection is less direct but noteworthy involving extracellular matrix proteins. Understanding FAM83H’s involvement in these disorders aids in the development of diagnostic and therapeutic strategies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

May play a major role in the structural organization and calcification of developing enamel (PubMed : 18252228). May play a role in keratin cytoskeleton disassembly by recruiting CSNK1A1 to keratin filaments. Thereby, it may regulate epithelial cell migration (PubMed : 23902688).
See full target information FAM83H
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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