Anti-FAM83G/PAWS1抗体(ab121750)
Key features and details
- Rabbit polyclonal to FAM83G/PAWS1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FAM83G/PAWS1抗体
参阅全部 FAM83G/PAWS1 一抗 -
描述
兔多克隆抗体to FAM83G/PAWS1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- RT4, U-251 MG, Human Liver and Human Tonsil lysates, Human oral mucosa tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121750于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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| WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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序列相似性
Belongs to the FAM83 family. - Information by UniProt
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数据库链接
- Entrez Gene: 644815 Human
- SwissProt: A6ND36 Human
- Unigene: 710727 Human
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别名
- FA83G_HUMAN antibody
- Fam83g antibody
- Family with sequence similarity 83, member G antibody
- Protein FAM83G antibody
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83G/PAWS1 antibody (ab121750)
ab121750 at 1/100 dilution staining FAM83G/PAWS1 in Paraffin-emebedded Human oral mucosa tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-FAM83G/PAWS1 antibody (ab121750)Immunocytochemistry/Immunofluorescence analysis of U-251 MG cells labelling FAM83G/PAWS1 with ab121750 shows positivity in the cytoplasm.
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Western blot - Anti-FAM83G/PAWS1 antibody (ab121750)All lanes : Anti-FAM83G/PAWS1 antibody (ab121750) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
10-20% SDS PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab121750 被引用在 6 文献中.
- Dunbar K et al. FAM83F regulates canonical Wnt signalling through an interaction with CK1a. Life Sci Alliance 4:N/A (2021). PubMed: 33361109
- Dunbar K et al. IMiDs induce FAM83F degradation via an interaction with CK1a to attenuate Wnt signalling. Life Sci Alliance 4:N/A (2021). PubMed: 33361334
- Okada J et al. FAM83G Is a Novel Inducer of Apoptosis. Molecules 25:N/A (2020). PubMed: 32570757
- Wu KZL et al. Pathogenic FAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1a association and attenuate Wnt signalling. Wellcome Open Res 4:133 (2019). PubMed: 31656861
- Maruthappu T et al. Recessive Mutation in FAM83G Associated with Palmoplantar Keratoderma and Exuberant Scalp Hair. J Invest Dermatol 138:984-987 (2018). IHC-P ; Human . PubMed: 29138053
- Bozatzi P et al. PAWS1 controls Wnt signalling through association with casein kinase 1a. EMBO Rep 19:N/A (2018). ICC/IF . PubMed: 29514862