重组Anti-FAM38A/PIEZO1抗体[EPR23826-103] (ab259949)
![Western blot - Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949)](https://www.abcam.cn/ps/products/259/ab259949/Images/ab259949-546668-anti-fam38a-piezo1-antibody-epr23826-103-western-blot-wildtype-hela-unboiled-piezo-knockout-hela-unboiled-a431-unboiled-thp.jpg "Western blot - Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23826-103] to FAM38A/PIEZO1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
概述
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产品名称
Anti-FAM38A/PIEZO1抗体[EPR23826-103]
参阅全部 FAM38A/PIEZO1 一抗 -
描述
兔单克隆抗体[EPR23826-103] to FAM38A/PIEZO1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type HeLa, unboiled A431 and THP-1, HeLa transfected with scrambled siRNA control whole cell lysate; A431 transfected with scrambled siRNA control, 25nM siRNA specifically targeting PIEZO1 or 100nM siRNA specifically targeting PIEZO1 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23826-103 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab259949于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 287 kDa.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 287 kDa. |
靶标
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功能
Component of mechanosensitive channel required for the mechanosensitive currents. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. -
组织特异性
Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. -
序列相似性
Belongs to the PIEZO family. -
细胞定位
Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9780 Human
- Omim: 611184 Human
- SwissProt: Q92508 Human
- Unigene: 377001 Human
- Unigene: 592074 Human
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别名
- DHS antibody
- Fam38a antibody
- Family with sequence similarity 38 member A antibody
see all
图片
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Western blot - Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949)All lanes : Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PIEZO1 knockout HeLa cell lysate
Lane 3 : A431 UNBOILED cell lysate
Lane 4 : THP-1 UNBOILED cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 287 kDa
Observed band size: 286 kDa why is the actual band size different from the predicted?Western blot: Anti-PIEZO1 antibody [EPR23826-103] (ab259949) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab259949 was shown to bind specifically to PIEZO1. A band was observed at 286 kDa in wild-type HeLa cell lysates with no signal observed at this size in PIEZO1 knockout cell line. To generate this image, wild-type and PIEZO1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949)](https://www.abcam.cn/ps/products/259/ab259949/Images/ab259949-470425-anti-fam38apiezo1-antibody-epr23826103-western-blot-adenocarcinoma.jpg)
Western blot - Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949)All lanes : Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 4.5 µg
Lane 2 : HeLa transfected with siRNA specifically targeting PIEZO1 whole cell lysate at 5 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 287 kDa
Observed band size: 300 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 29396846).
Exposure time: 74 seconds.
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![Western blot - Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949)](https://www.abcam.cn/ps/products/259/ab259949/Images/ab259949-470426-anti-fam38apiezo1-antibody-epr23826103-western-blot-carcinoma.jpg)
Western blot - Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949)All lanes : Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma epithelial cell), transfected with scrambled siRNA control whole cell lysate
Lane 2 : A431 transfected with 25nM siRNA specifically targeting PIEZO1 whole cell lysate
Lane 3 : A431 transfected with 100nM siRNA specifically targeting PIEZO1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 287 kDa
Observed band size: 300 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 29396846).
Exposure time: 26 seconds.
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![Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949)](https://www.abcam.cn/ps/products/259/ab259949/Images/ab259949-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (ab259949)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab259949 尚未被引用在任何文献中。