Anti-FAM190B抗体(ab122393)
Key features and details
- Rabbit polyclonal to FAM190B
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-FAM190B抗体 -
描述
兔多克隆抗体to FAM190B -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
internal sequence amino acids 568-664 (NVECDNMNRF DRPDRNVRQP QEGFWKRPPQ RWSGQEHYHL SHPDHYHHHG KSDLSRGSPY RESPLGHFES YGGMPFFQAQ KMFVDVPENT VILDEMT) of the Human FAM190B protein (Q9H7U1).
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阳性对照
- IHC-P: Human adrenal gland tissue WB: RT4, U251 MG, Human Liver and Tonsil cell lysates (weak bands)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122393于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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序列相似性
Belongs to the FAM190 family. - Information by UniProt
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数据库链接
- Entrez Gene: 54462 Human
- Entrez Gene: 72972 Mouse
- Entrez Gene: 306306 Rat
- SwissProt: Q9H7U1 Human
- SwissProt: Q3UHI0 Mouse
- Unigene: 461988 Human
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别名
- F190B_HUMAN antibody
- FAM190B antibody
- KIAA1128 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-FAM190B antibody (ab122393)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122393 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Western blot - Anti-FAM190B antibody (ab122393)Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM190B antibody (ab122393)ab122393, at 1/500, staining FAM190B in paraffin embedded adrenal gland tissue by immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab122393 被引用在 1 文献中.
- Mun DJ et al. Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment. Proc Natl Acad Sci U S A 120:e2214507120 (2023). PubMed: 36795749