Anti-FAM162A抗体(ab122295)
Key features and details
- Rabbit polyclonal to FAM162A
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FAM162A抗体 -
描述
兔多克隆抗体to FAM162A -
宿主
Rabbit -
经测试应用
适用于: ICC, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human FAM162A.
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阳性对照
- WB: U-87 MG cell line lysate; IHC-P: Human rectum, cerebral cortex, and testis tissues; ICC: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122295于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 0.25 - 2 µg/ml.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
ICC
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Belongs to the UPF0389 family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 26355 Human
- Omim: 608017 Human
- SwissProt: Q96A26 Human
- Unigene: 584881 Human
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别名
- C3orf28 antibody
- Chromosome 3 open reading frame 28 antibody
- DC16 antibody
see all
图片
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Western blot - Anti-FAM162A antibody (ab122295)Anti-FAM162A antibody (ab122295) at 0.4 µg/ml + U-87 MG cell
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM162A antibody (ab122295)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human rectum tissue labelling FAM162A with ab122295 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-FAM162A antibody (ab122295)Immunocytochemistry analysis of A-431 cells labelling FAM162A with ab122295 shows positivity in the mitochondria.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM162A antibody (ab122295)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling FAM162A with ab122295 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM162A antibody (ab122295)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling FAM162A with ab122295 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab122295 被引用在 1 文献中.
- Zhao XH et al. Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients. iScience 26:106831 (2023). PubMed: 37250791