Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-FADD (phospho S194)抗体(ab55334)

Reviews (5)Q&A (2)
Western blot - Anti-FADD (phospho S194) antibody (ab55334)

    Key features and details

    • Rabbit polyclonal to FADD (phospho S194)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-FADD (phospho S194)抗体
      参阅全部 FADD 一抗

    • 描述

      兔多克隆抗体to FADD (phospho S194)

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse

    • 免疫原

      Synthetic peptide corresponding to Human FADD aa 150-250 (phospho S194).
      Database link: Q13158

    • 阳性对照

      • Extracts from HeLa cells.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab55334于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (5)
    1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).
    说明
    WB
    1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).

    靶标

    • 功能

      Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.

    • 组织特异性

      Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.

    • 序列相似性

      Contains 1 death domain.
      Contains 1 DED (death effector) domain.

    • 结构域

      Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
    • Information by UniProt

    • 数据库链接

      • 别名

        • FADD antibody
        • FADD protein antibody
        • FADD_HUMAN antibody
        • Fas (TNFRSF6) associated via death domain antibody
        • Fas associated via death domain antibody
        • Fas associating death domain containing protein antibody
        • Fas associating protein antibody
        • Fas associating protein with death domain antibody
        • Fas TNFRSF6 associated via death domain antibody
        • FAS-associated death domain protein antibody
        • FAS-associating death domain-containing protein antibody
        • GIG 3 antibody
        • GIG3 antibody
        • Growth inhibiting gene 3 protein antibody
        • Growth-inhibiting gene 3 protein antibody
        • H sapiens mRNA for mediator of receptor induced toxicity antibody
        • Mediator of receptor induced toxicity antibody
        • MGC8528 antibody
        • MORT 1 antibody
        • MORT1 antibody
        • Protein FADD antibody
        see all

      图片

      • Western blot - Anti-FADD (phospho S194) antibody (ab55334)
        Western blot - Anti-FADD (phospho S194) antibody (ab55334)
        All lanes : Anti-FADD (phospho S194) antibody (ab55334) at 1/500 dilution

        Lane 1 : Extracts from HeLa cells treated
        with Paclitaxel (1µM, 60mins).
        Lane 2 : Extracts from HeLa cells treated
        with Paclitaxel (1µM, 60mins), and with the immunising phosphpeptide.

        Predicted band size: 23 kDa
        Observed band size: 30 kDa why is the actual band size different from the predicted?

      文献 (0)

      发表研究结果有使用 ab55334？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab55334 尚未被引用在任何文献中。

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-7 of 7 Abreviews or Q&A

      Western blot abreview for Anti-FADD (phospho S194) antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
      Loading amount
      0.5 µg
      Specification
      Rabbit Reticulocyte
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Dec 01 2016

      Western blot abreview for Anti-FADD (phospho S194) antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Hamster Cell lysate - whole cell (BHK-21 (Kidney Fibroblasts))
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
      Loading amount
      500000 cells
      Specification
      BHK-21 (Kidney Fibroblasts)
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Oct 31 2016

      Western blot abreview for Anti-FADD (phospho S194) antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
      Loading amount
      500000 cells
      Specification
      Canine Kidney Epithelial MDCK cells
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Oct 21 2016

      Western blot abreview for Anti-FADD (phospho S194) antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human Foreskin Fibroblasts)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
      Loading amount
      200000 cells
      Specification
      Human Foreskin Fibroblasts
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Feb 09 2016

      Western blot abreview for Anti-FADD (phospho S194) antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (mouse embryonic fibroblasts (MEFs))
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
      Loading amount
      200000 cells
      Specification
      mouse embryonic fibroblasts (MEFs)
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Feb 09 2016

      Question

      Please send a testing discount code for tesing reactivity of ab55334 with mouse samples.

      Read More
      Answer

      I am very pleased to hear you would like to accept our offer and test ab55334 on mouse samples by western blotting. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview of mouse reactivity and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.


      For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Question

      I look into your data sheet cannot find reference/pdfs for these antibodies:

      ab122926
      ab55334
      ab59425
      ab28820

      We areinterested inpublications for experiences example with those antibodies (WB, IHC, IF).

      Thank you very much for your help!

      Read More
      Answer

      Thank you for your inquiry.


      If we know of our antibodies being used in any references/publications, we will list these under the "Specific References" section of our datasheet, which can be found under the shipping and pricing information online.


      We know of one reference for ab112926: Okamoto K et al. DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation. Mol Cell Biol 25:9608-20 (2005). PubMed: 16227609


      https://www.abcam.com/MDMX-phospho-S367-antibody-15-ab122926-references.html


      We do not know of any publications for ab55334, ab59425, or ab28820. We would still guarantee these antibodies to work as stated on the datasheets in accordance with our Abpromise.


      I hope this information helps. Please contact us with any other questions.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com