Name: Anti-FADD antibody [OTI1C11]
SKU: ab119059
Rating: 5 (3 reviews)

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Western blot - Anti-FADD antibody [OTI1C11] (ab119059)

Key features and details

Mouse monoclonal [OTI1C11] to FADD
Suitable for: WB, Flow Cyt (Intra)
Knockout validated
Reacts with: Human, Recombinant fragment
Isotype: IgG1

产品名称

Anti-FADD抗体[OTI1C11]
参阅全部 FADD 一抗
描述

小鼠单克隆抗体[OTI1C11] to FADD
宿主

Mouse
经测试应用

适用于: WB, Flow Cyt (Intra)more details
种属反应性

与反应: Human, Recombinant fragment
免疫原

Recombinant full length protein corresponding to Human FADD. Protein produced in HEK293T cells
Database link: NP_003815
阳性对照
- WB: HEK-293T cells transfected with pCMV6-ENTRY FADD cDNA, A431, HepG2, HeLa, A549, MCF7 and Jurkat cell lysates. Flow Cyt (Intra): HEK-293T cells transfected with a FADD overexpression plasmid.
常规说明

The clone number has been updated from 1C11 to OTI1C11, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS
Concentration information loading...
纯度

Affinity purified
纯化说明

Purified from cell culture supernatant by affinity chromatography
克隆

单克隆
克隆编号

OTI1C11
同种型

IgG1
研究领域

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
KO cell lines
- Human FADD knockout HeLa cell line (ab261817)
KO cell lysates
- Human FADD knockout HeLa cell lysate (ab257261)
Recombinant Protein
- Recombinant Human FADD protein (ab89242)

The Abpromise guarantee

Abpromise™承诺保证使用ab119059于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(3)	1/2000. Predicted molecular weight: 23 kDa.
Flow Cyt (Intra)		1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

说明
WB 1/2000. Predicted molecular weight: 23 kDa.
Flow Cyt (Intra) 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

功能

Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
组织特异性

Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
序列相似性

Contains 1 death domain.
Contains 1 DED (death effector) domain.
结构域

Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
Target information above from: UniProt accession Q13158 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 8772 Human
- Omim: 602457 Human
- SwissProt: Q13158 Human
- Unigene: 86131 Human
别名
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
- Fas (TNFRSF6) associated via death domain antibody
- Fas associated via death domain antibody
- Fas associating death domain containing protein antibody
- Fas associating protein antibody
- Fas associating protein with death domain antibody
- Fas TNFRSF6 associated via death domain antibody
- FAS-associated death domain protein antibody
- FAS-associating death domain-containing protein antibody
- GIG 3 antibody
- GIG3 antibody
- Growth inhibiting gene 3 protein antibody
- Growth-inhibiting gene 3 protein antibody
- H sapiens mRNA for mediator of receptor induced toxicity antibody
- Mediator of receptor induced toxicity antibody
- MGC8528 antibody
- MORT 1 antibody
- MORT1 antibody
- Protein FADD antibody
see all

Western blot - Anti-FADD antibody [OTI1C11] (ab119059)

All lanes : Anti-FADD antibody [OTI1C11] (ab119059) at 1/2000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FADD knockout HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 23 kDa

Lanes 1-4: Merged signal (red and green). Green - ab119059 observed at 25 kDa. Red - loading control ab181602 observed at 37 kDa.

ab119059 Anti-FADD antibody [OTI1C11] was shown to specifically react with FADD in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261817 (knockout cell lysate ab257261) was used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab119059 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-FADD antibody [OTI1C11] (ab119059)

All lanes : Anti-FADD antibody [OTI1C11] (ab119059) at 1/2000 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : FADD knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 23 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab119059 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab119059 was shown to specifically react with FADD when FADD knockout samples were used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab119059 and ab181602 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-FADD antibody [OTI1C11] (ab119059)

All lanes : Anti-FADD antibody [OTI1C11] (ab119059) at 1/2000 dilution

Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY FADD cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 23 kDa
Western blot - Anti-FADD antibody [OTI1C11] (ab119059)

All lanes : Anti-FADD antibody [OTI1C11] (ab119059) at 1/200 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 23 kDa
Flow Cytometry (Intracellular) - Anti-FADD antibody [OTI1C11] (ab119059)

HEK293T cells transfected with either a FADD overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab119059 at 1/100 dilution and then analyzed by Flow Cytometry (Intracellular).

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Language
Datasheet download

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发表研究结果有使用 ab119059？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab119059 被引用在 1 文献中.

Stöhr D et al. Stress-induced TRAILR2 expression overcomes TRAIL resistance in cancer cell spheroids. Cell Death Differ 27:3037-3052 (2020). PubMed: 32433558

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1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

African Green Monkey Cell lysate - whole cell (VERO E6)

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)

Loading amount

75000 cells

Specification

VERO E6

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 15 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (Human Foreskin Fibroblasts)

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)

Loading amount

50000 cells

Treatment

Infected with human cytomegalovirus for 72 hrs

Specification

Human Foreskin Fibroblasts

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 13 2016

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts (MEFs))

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)

Loading amount

25000 cells

Specification

Mouse embryonic fibroblasts (MEFs)

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 13 2016

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Key features and details

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

纯化说明

克隆

克隆编号

同种型

研究领域

相关产品

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

结构域

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (1)

客户评价及客户问答

Western blot abreview for Anti-FADD antibody [1C11]

Western blot abreview for Anti-FADD antibody [1C11]

Western blot abreview for Anti-FADD antibody [1C11]