Anti-Factor XIIIa抗体[AC-1A1] (ab1834)
Key features and details
- Mouse monoclonal [AC-1A1] to Factor XIIIa
- Suitable for: ELISA, ICC/IF, IHC-Fr, IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-Factor XIIIa抗体[AC-1A1]
参阅全部 Factor XIIIa 一抗 -
描述
小鼠单克隆抗体[AC-1A1] to Factor XIIIa -
宿主
Mouse -
经测试应用
适用于: ELISA, ICC/IF, IHC-Fr, IHC-P, WB, Flow Cytmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Factor XIIIa. BALB/C mice were injected with recombinant human protein corresponding to A-subunit of coagulation Factor XIII.
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阳性对照
- Placenta
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常规说明
This antibody recognizes both the dimer and monomer forms.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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纯度
IgG fraction -
克隆
单克隆 -
克隆编号
AC-1A1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
This antibody may be diluted to a titer of 1/25 - 1/50 in an ABC method. |
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. This antibody may be diluted to a titer of 1/25 - 1/50 in an ABC method. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. -
疾病相关
Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. -
序列相似性
Belongs to the transglutaminase superfamily. Transglutaminase family. -
翻译后修饰
The activation peptide is released by thrombin. -
细胞定位
Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma. - Information by UniProt
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数据库链接
- Entrez Gene: 2162 Human
- Entrez Gene: 74145 Mouse
- Omim: 134570 Human
- SwissProt: P00488 Human
- SwissProt: Q8BH61 Mouse
- Unigene: 335513 Human
- Unigene: 235105 Mouse
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别名
- bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
- Coagulation factor XIII A chain antibody
- Coagulation factor XIII A1 polypeptide antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)
ab1834 - immunohistochemistry
Formalin fixed paraffin embedded human placenta stained with Factor XIIIa, using ABC and AEC chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)ab1834 staining human placenta by IHC-P.
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Anti-Factor XIIIa antibody [AC-1A1] (ab1834) at 1/250 dilution + Human placenta tissue lysate - total protein at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Additional bands at: 37 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Factor XIIIa with ab1834.
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Overlay histogram showing A549 cells stained with ab16956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab1834 被引用在 14 文献中.
- Hu PA et al. New Mechanisms of Bromelain in Alleviating Non-Alcoholic Fatty Liver Disease-Induced Deregulation of Blood Coagulation. Nutrients 14:N/A (2022). PubMed: 35684129
- Piliponsky AM et al. Mast cell-derived factor XIIIA contributes to sexual dimorphic defense against group B streptococcal infections. J Clin Invest 132:N/A (2022). PubMed: 36006736
- Ercan H et al. Alterations of the Platelet Proteome in Lung Cancer: Accelerated F13A1 and ER Processing as New Actors in Hypercoagulability. Cancers (Basel) 13:N/A (2021). PubMed: 34066760
- Ercan H et al. Platelet Phenotype Analysis of COVID-19 Patients Reveals Progressive Changes in the Activation of Integrin αIIbβ3, F13A1, the SARS-CoV-2 Target EIF4A1 and Annexin A5. Front Cardiovasc Med 8:779073 (2021). PubMed: 34859078
- Li C et al. Potential Markers from Serum-Purified Exosomes for Detecting Oral Squamous Cell Carcinoma Metastasis. Cancer Epidemiol Biomarkers Prev 28:1668-1681 (2019). PubMed: 31350263