AB242123

重组Anti-Factor XII抗体[EPR22292-127]

Anti-Factor XII antibody [EPR22292-127]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal Factor XII antibody. Suitable for WB, IP and reacts with Recombinant full length protein - Human, Human samples. Cited in 4 publications.

查看别名

Coagulation factor XII, Hageman factor, HAF, F12

5 Images

Supplier Data

Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] (AB242123)

Factor XII was immunoprecipitated from 0.35 mg of human plasma with ab242123 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242123 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Human plasma 10 μg (Input).

Lane 2 : ab242123 IP in human plasma.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab242123 in human plasma.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

Factor XII is an 80kDa glycoprotein undergoes catalysis and forms the 70kDa cleavage and 50kDa heavy chain of alpha-factor XIIa. (PMID : 8049433, PMID 2473984).

All lanes:

Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] (ab242123)

Predicted band size: 67 kDa

false

Supplier Data

Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] (AB242123)

Factor XII was immunoprecipitated from 0.35 mg of human serum with ab242123 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242123 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Human serum 10 μg (Input).

Lane 2 : ab242123 IP in human serum.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab242123 in human serum.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

Factor XII is an 80kDa glycoprotein undergoes catalysis and forms the 70kDa cleavage and 50kDa heavy chain of alpha-factor XIIa. (PMID : 8049433, PMID 2473984).

All lanes:

Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] (ab242123)

Predicted band size: 25 kDa,67 kDa

false

Supplier Data

Western blot - Anti-Factor XII antibody [EPR22292-127] (AB242123)

Exposure time : Lane 1 : 8 Seconds; Lane 2 : 3 minutes.

Blocking/Dilution buffer : 5% NFDM/TBST.

Factor XII is an 80-kDa polypeptide undergoes catalysis and forms the 50-kDa heavy and 30-kDa light chains of alpha-factor XIIa. The molecular weight observed is consistent with literature (PMID : 28069606; 2473984; 8049433).

All lanes:

Western blot - Anti-Factor XII antibody [EPR22292-127] (ab242123) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human serum at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 67 kDa

Observed band size: 50 kDa,80 kDa

false

Lab

Western blot - Anti-Factor XII antibody [EPR22292-127] (AB242123)

In Western blot, Anti-Anti-6X His tag® antibody [AD1.1.10] loading control staining at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human light chain Factor XII.

All lanes:

Western blot - Anti-Factor XII antibody [EPR22292-127] (ab242123) at 1/1000 dilution

Lane 1:

His-tagged heavy chain of human Factor XII recombinant protein at 10 ng

Lane 2:

Lif/His-tagged light chain of human Factor XII recombinant protein at 10 ng

Lane 3:

No-tagged human Factor XII recombinant protein without His tag at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 65 kDa,50-70 kDa

false

Exposure time: 92s

Supplier Data

Western blot - Anti-Factor XII antibody [EPR22292-127] (AB242123)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Factor XII antibody [EPR22292-127] (ab242123) at 1/1000 dilution

All lanes:

Western blot - Native Human Factor XII protein (<a href='/products/proteins-peptides/native-human-factor-xii-protein-ab62423'>ab62423</a>) at 20 µg/mL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 67 kDa

Observed band size: 50 kDa,80 kDa

false

Exposure time: 3min

不同偶联物与剂型 (1)

Carrier free

Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22292-127

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody does not cross-react with human light chain Factor XII.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Factor XII also known as FXII or Hageman factor is a serine protease and a coagulation factor that plays an important role in initiating the intrinsic pathway of blood coagulation. It has a molecular mass of approximately 80 kDa. The liver primarily synthesizes this protein and it circulates in the blood plasma as an inactive precursor. When activated to Factor XIIa it cleaves and activates other coagulation factors such as Factor XI to propagate the coagulation cascade that helps in blood clot formation.

Biological function summary

This protein helps control blood coagulation processes as part of a larger network of interacting proteins that includes contact activation factors. Factor XII activates not only the coagulation cascade but also pathways involved in fibrinolysis inflammation and bradykinin formation. It triggers the kallikrein-kinin system and leads to the generation of bradykinin a peptide known to induce vascular permeability and inflammation.

Pathways

The involvement of Factor XII spans the intrinsic coagulation pathway and the kallikrein-kinin system. It interacts with several other coagulation factors such as Factor XI and prekallikrein to advance the clotting process. The protein initiates the intrinsic blood coagulation pathway by converting Factor XI to Factor XIa in the presence of negatively charged surfaces. It links to the fibrinolytic pathway and can indirectly activate plasminogen through interactions that generate plasmin.

Factor XII deficiency leads to increased risk of thrombosis even though such deficiency generally shows no major bleeding symptoms. The target links to hereditary angioedema where dysregulation in the kallikrein-kinin system involves premature bradykinin activity. Factor XII associates with C1-inhibitor a protein playing a regulatory role in preventing excessive bradykinin production which can manifest in clinical symptoms seen in hereditary angioedema patients.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (PubMed : 2019570, PubMed : 21304106, PubMed : 8427954).

See full target information F12

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文献 (4)

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2024

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ACS applied materials & interfaces 16:4307-4320 PubMed38240181

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Yibing Dong,Yaoxian Xu,Chengxing Lian,Krisna Prak,Hwa Liang Leo,Teresa D Tetley,Vania Braga,Mike Emerson,Josefin Ahnström,Choon Hwai Yap

Cell research 30:119-132 PubMed31811276

2019

Transferrin plays a central role in coagulation balance by interacting with clotting factors.

Applications

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Species

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Xiaopeng Tang,Zhiye Zhang,Mingqian Fang,Yajun Han,Gan Wang,Sheng Wang,Min Xue,Yaxiong Li,Li Zhang,Jian Wu,Biqing Yang,James Mwangi,Qiumin Lu,Xiaoping Du,Ren Lai

View all publications

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重组Anti-Factor XII抗体[EPR22292-127]