Anti-Factor VIII抗体[GMA-012] (ab78852)
![Western blot - Anti-Factor VIII antibody [GMA-012] (ab78852)](https://www.abcam.cn/ps/products/78/ab78852/Images/Factor-VIII-Primary-antibodies-ab78852-16.jpg "Western blot - Anti-Factor VIII antibody [GMA-012] (ab78852)")
Key features and details
- Mouse monoclonal [GMA-012] to Factor VIII
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Factor VIII抗体[GMA-012]
参阅全部 Factor VIII 一抗 -
描述
小鼠单克隆抗体[GMA-012] to Factor VIII -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Purified human Factor VIII
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阳性对照
- Human Factor VIII. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituents: 1% Mannitol, 0.87% Sodium chloride, 0.164% Sodium phosphate -
Concentration information loading... -
纯度
DEAE-Chromatography -
克隆
单克隆 -
克隆编号
GMA-012 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78852于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 280 kDa (predicted molecular weight: 267 kDa).
|
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 280 kDa (predicted molecular weight: 267 kDa). |
|
IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. -
疾病相关
Defects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. -
序列相似性
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 2 F5/8 type C domains.
Contains 6 plastocyanin-like domains. -
结构域
Domain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity. -
翻译后修饰
Sulfation on Tyr-1699 is essential for binding vWF. -
细胞定位
Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 2157 Human
- Omim: 300841 Human
- SwissProt: P00451 Human
- Unigene: 654450 Human
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别名
- AHF antibody
- Antihemophilic factor antibody
- Coagulation factor VIII antibody
see all
图片
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Western blot - Anti-Factor VIII antibody [GMA-012] (ab78852)Anti-Factor VIII antibody [GMA-012] (ab78852) at 1 µg/ml + human Factor VIII at 2 µg
Secondary
Goat anti-mouse HRP conjugated IgG
Predicted band size: 267 kDa
Observed band size: 280 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [GMA-012] (ab78852)](https://www.abcam.cn/ps/products/78/ab78852/Images/Factor-VIII-Primary-antibodies-ab78852-17.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [GMA-012] (ab78852)IHC image of Factor VIII staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78852, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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Datasheet download
文献 (4)
ab78852 被引用在 4 文献中.
- Gong X et al. Effect of heme oxygenase 1 and renin/prorenin receptor on oxidized low-density lipoprotein-induced human umbilical vein endothelial cells. Exp Ther Med 18:1752-1760 (2019). PubMed: 31410134
- Ye W et al. Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA-Meg3/miR-421/PDGFRA axis. Int J Oncol 55:1296-1312 (2019). PubMed: 31638182
- Yu J et al. Homocysteine inhibits the viability and migration ability of human umbilical vein endothelial cells by downregulating the expression of vascular endothelial growth factor. Exp Ther Med 18:3913-3919 (2019). PubMed: 31641378
- Tian X et al. ß-Caryophyllene protects in vitro neurovascular unit against oxygen-glucose deprivation and re-oxygenation-induced injury. J Neurochem 139:757-768 (2016). PubMed: 27565895
