Anti-Factor VII抗体(ab97614)
Key features and details
- Rabbit polyclonal to Factor VII
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Factor VII抗体
参阅全部 Factor VII 一抗 -
描述
兔多克隆抗体to Factor VII -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Monkey
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免疫原
Recombinant protein fragment corresponding to a region within amino acids 209 and 444 of Human Factor VII.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97614于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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| IHC-P |
1/100 - 1/500.
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| 说明 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
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IHC-P
1/100 - 1/500. |
靶标
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功能
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. -
组织特异性
Plasma. -
疾病相关
Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. FA7D is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level. -
序列相似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
翻译后修饰
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2155 Human
- Entrez Gene: 14068 Mouse
- Omim: 613878 Human
- SwissProt: P08709 Human
- SwissProt: P70375 Mouse
- Unigene: 36989 Human
- Unigene: 4827 Mouse
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别名
- coagulation factor VII (serum prothrombin conversion accelerator) antibody
- Coagulation factor VII antibody
- Eptacog alfa antibody
see all
图片
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Western blot - Anti-Factor VII antibody (ab97614)Anti-Factor VII antibody (ab97614) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS PAGE -
Western blot - Anti-Factor VII antibody (ab97614)Anti-Factor VII antibody (ab97614) at 1/500 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 52 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VII antibody (ab97614)Immunohistochemistry analysis of paraffin-embedded N human (gastric carcinoma) tissue, using ab97614 at 1/100 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab97614 被引用在 8 文献中.
- Wypasek E et al. Effects of rivaroxaban and dabigatran on local expression of coagulation and inflammatory factors within human aortic stenotic valves. Vascul Pharmacol 130:106679 (2020). PubMed: 32387621
- Mao XB et al. miR-204-5p promotes diabetic retinopathy development via downregulation of microtubule-associated protein 1 light chain 3. Exp Ther Med 17:2945-2952 (2019). PubMed: 30936964
- Mashayekhi A et al. Functional and Molecular Characterization of C91S Mutation in the Second Epidermal Growth Factor-Like Domain of Factor VII. Iran J Biotechnol 16:e1813 (2018). PubMed: 30555841
- Mashayekhi A et al. In vitro expression of mutant factor VII proteins and characterization of their clinical significance. Mol Med Rep 17:2738-2742 (2018). PubMed: 29207137
- Zhang S et al. Synergistic Inhibitory Effect of Traditional Chinese Medicine Astragaloside IV and Curcumin on Tumor Growth and Angiogenesis in an Orthotopic Nude-Mouse Model of Human Hepatocellular Carcinoma. Anticancer Res 37:465-473 (2017). PubMed: 28179291
- Huang KT et al. Factor VII-Induced MicroRNA-135a Inhibits Autophagy and Is Associated with Poor Prognosis in Hepatocellular Carcinoma. Mol Ther Nucleic Acids 9:274-283 (2017). PubMed: 29246306
- Nagahashi K et al. Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells. Cytotechnology 69:317-327 (2017). PubMed: 28070807
- Chen KD et al. Interconnections between autophagy and the coagulation cascade in hepatocellular carcinoma. Cell Death Dis 5:e1244 (2014). WB ; Human . PubMed: 24853422