JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB108614

重组Anti-Factor V抗体[EPR5191]

Anti-Factor V antibody [EPR5191]

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Recombinant Monoclonal Factor V antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

查看别名

Coagulation factor V, Activated protein C cofactor, F5

1 Images
Western blot - Anti-Factor V antibody [EPR5191] (AB108614)
  • WB

Unknown

Western blot - Anti-Factor V antibody [EPR5191] (AB108614)

All lanes:

Western blot - Anti-Factor V antibody [EPR5191] (ab108614) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human platelet lysate at 10 µg

Predicted band size: 252 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-Factor V antibody [EPR5191] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5191

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
存储溶液
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Factor V also known as proaccelerin is a coagulation factor playing a main role in blood clotting. This protein with a molecular mass of approximately 330 kDa is synthesized in the liver and found in plasma. The Factor V protein is essential for the coagulation cascade functioning as a pivotal mediator in the conversion of prothrombin to thrombin. It exists in plasma in an inactive form until activated by thrombin or factor Xa to fulfill its mechanical function in hemostasis.
Biological function summary

The mechanism of Factor V involves its function as a cofactor to Factor Xa in the prothrombinase complex. This complex formation enhances the efficiency of thrombin generation considerably accelerating clot formation. Factor V once converted to its active form aids in forming a stable clot by bridging and immobilizing cellular elements and fibrin strands. Expression occurs mainly in the liver but it also manifests in platelets where it enhances thrombin production during platelet activation and aggregation.

Pathways

The contribution of Factor V to coagulation is central within the hemostasis pathway specifically the intrinsic and extrinsic pathways of the coagulation cascade. Factor V binds with Factor X to form the prothrombinase complex which is important for the conversion of prothrombin to thrombin. Other proteins such as Factor VIII interact within this pathway to ensure blood clots form properly. Any disturbances in Factor V activity can significantly alter the normal flow of these pathways affecting thrombin generation and subsequent clotting.

Factor V mutations or deficiencies associate with conditions like Factor V Leiden and Factor V deficiency. Factor V Leiden is a genetic mutation that elevates the risk of thrombophilia leading to increased clot formation in veins. On the other hand a deficiency in Factor V can lead to Parahemophilia characterized by a tendency for excessive bleeding. In both conditions the abnormal activity of Factor V affects the interaction with proteins in the coagulation cascade especially impacting the prothrombinase complex and Factor X.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
See full target information F5
style
left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:2404 PubMed38493152

2024

Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Watanabe,Yoshiaki Kise,Kento Yonezawa,Mariko Inoue,Nobutaka Shimizu,Osamu Nureki,Kenji Inaba

Transfusion 60:401-413 PubMed31785023

2019

Clinical manifestation of hemophilia A in the absence of mutations in the F8 gene that encodes FVIII: role of microRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna I Jankowska,Joseph McGill,Behnaz Pezeshkpoor,Johannes Oldenburg,Chintamani D Atreya,Zuben E Sauna

Nature communications 10:3224 PubMed31324782

2019

Active thrombin produced by the intestinal epithelium controls mucosal biofilms.

Applications

WB

Species

Human

Jean-Paul Motta,Alexandre Denadai-Souza,David Sagnat,Laura Guiraud,Anissa Edir,Chrystelle Bonnart,Mireille Sebbag,Perrine Rousset,Ariane Lapeyre,Carine Seguy,Noa Mathurine-Thomas,Heather J Galipeau,Delphine Bonnet,Laurent Alric,Andre G Buret,John L Wallace,Antoine Dufour,Elena F Verdu,Morley D Hollenberg,Eric Oswald,Matteo Serino,Celine Deraison,Nathalie Vergnolle

Cancer gene therapy 23:303-14 PubMed27492853

2016

Retargeted adenoviruses for radiation-guided gene delivery.

Applications

Flow Cyt

Species

Human

S A Kaliberov,L N Kaliberova,H Yan,V Kapoor,D E Hallahan
View all publications
style
left-column
style
left-column
style
right-column

替代产品

Primary Antibodies

AB48230

Biotin Anti-Factor V antibody

  • 0
  • 0
Primary Antibodies

AB234849

Anti-Factor V antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor V antibody (AB234849)

  • 0
  • 0

搭配使用产品

Assay Kits

AB137976

Human Factor V ELISA Kit

Sandwich ELISA - Human Factor V ELISA Kit (AB137976)

  • 0
  • 0
Primary Antibodies

AB92621

Anti-Thrombin antibody

Western blot - Anti-Thrombin antibody (AB92621)

  • 4
  • 5
Primary Antibodies

AB196023

Anti-Factor X antibody [EPR16249]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor X antibody [EPR16249] (AB196023)

  • 0
  • 0
Primary Antibodies

AB289971

Anti-HC-II antibody [EPR25618-1]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-HC-II antibody [EPR25618-1] (AB289971)

  • 0
  • 0
Primary Antibodies

AB255824

Anti-Factor IX/PTC antibody [EPR22846-238]

RabMAb|Recombinant

Immunoprecipitation - Anti-Factor IX/PTC antibody [EPR22846-238] (AB255824)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com