重组Anti-Factor IX/PTC抗体[EPR28007-19] - BSA and Azide free (ab318269)
![Western blot - Anti-Factor IX/PTC antibody [EPR28007-19] - BSA and Azide free (ab318269)](https://www.abcam.cn/ps/products/318/ab318269/Images/ab318269-545275-anti-factor-ixptc-antibody-epr28007-19-western-blot-mouse-plasma-lysate-20-ug.jpg "Western blot - Anti-Factor IX/PTC antibody [EPR28007-19] - BSA and Azide free (ab318269)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28007-19] to Factor IX/PTC - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-Factor IX/PTC抗体[EPR28007-19] - BSA and Azide free
参阅全部 Factor IX/PTC 一抗 -
描述
兔单克隆抗体[EPR28007-19] to Factor IX/PTC - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse
不与反应: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse plasma and Mouse liver lysates. IHC-P: Mouse perfused fixed liver, Mouse non-perfused fixed liver, Mouse non-perfused fixed cardiac muscle and Mouse perfused fixed skeletal muscle tissues.
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常规说明
ab318269 is the carrier-free version of ab318268
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28007-19 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. -
组织特异性
Synthesized primarily in the liver and secreted in plasma. -
疾病相关
Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis. -
序列相似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
结构域
Calcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain. -
翻译后修饰
Activated by factor XIa, which excises the activation peptide.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 14071 Mouse
- SwissProt: P16294 Mouse
- Unigene: 391283 Mouse
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别名
- Christmas Disease antibody
- Christmas factor antibody
- Coagulant factor IX antibody
see all
图片
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Western blot - Anti-Factor IX/PTC antibody [EPR28007-19] - BSA and Azide free (ab318269)All lanes : Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268) at 1/1000 dilution
Lane 1 : Mouse plasma lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lane 4 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 37,75 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThis data was developed using ab318268, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: brain, skeletal muscle(PMID:2323576)
Lane4 was developed using a higher sensitivity ECL substrate.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] - BSA and Azide free (ab318269)](https://www.abcam.cn/ps/products/318/ab318269/Images/ab318269-2-anti-factor-ixptc-antibody-epr28007-19-immunohistochemistry-a-mouse-perfused-f.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] - BSA and Azide free (ab318269)This data was developed using ab318268, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Mouse perfused fixed liver. (B) Mouse non-perfused fixed liver. tissue labeling Factor IX/PTC with ab318268 at 1/100 (4.91 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Positive staining in both (A) mouse perfused fixed liver and (B) mouse non-perfused fixed liver.
The section was incubated with ab318268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] - BSA and Azide free (ab318269)](https://www.abcam.cn/ps/products/318/ab318269/Images/ab318269-3-anti-factor-ixptc-antibody-epr28007-19-immunohistochemistry-a-mouse-perfused-f1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] - BSA and Azide free (ab318269)This data was developed using ab318268, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Mouse perfused fixed cardiac muscle. (B) Mouse non-perfused fixed cardiac muscle. tissue labeling Factor IX/PTC with ab318268 at 1/100 (4.91 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Nearly no staining in (A) mouse perfused fixed cardiac muscle; positive staining in plasma of (B) mouse non-perfused fixed cardiac muscle.
The section was incubated with ab318268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] - BSA and Azide free (ab318269)](https://www.abcam.cn/ps/products/318/ab318269/Images/ab318269-4-anti-factor-ixptc-antibody-epr28007-19-immunohistochemistry-mouse-perfused-fixed.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] - BSA and Azide free (ab318269)This data was developed using ab318268, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse perfused fixed skeletal muscle tissue labeling Factor IX/PTC with ab318268 at 1/100 (4.91 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Negative control: No staining in mouse perfused fixed skeletal muscle.
The section was incubated with ab318268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Anti-Factor IX/PTC antibody [EPR28007-19] - BSA and Azide free (ab318269)](https://www.abcam.cn/ps/products/318/ab318269/Images/ab318269-5-benefitsofrecombinantantibodies.jpg)
Anti-Factor IX/PTC antibody [EPR28007-19] - BSA and Azide free (ab318269)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab318269 尚未被引用在任何文献中。