重组Anti-Factor IX/PTC抗体[EPR28007-19] (ab318268)
![Western blot - Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268)](https://www.abcam.cn/ps/products/318/ab318268/Images/ab318268-545257-anti-factor-ixptc-antibody-epr28007-19-western-blot-mouse-plasma-lysate-20-ug.jpg "Western blot - Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28007-19] to Factor IX/PTC
- Suitable for: IHC-P, WB
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-Factor IX/PTC抗体[EPR28007-19]
参阅全部 Factor IX/PTC 一抗 -
描述
兔单克隆抗体[EPR28007-19] to Factor IX/PTC -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse
不与反应: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse plasma and Mouse liver lysates. IHC-P: Mouse perfused fixed liver, Mouse non-perfused fixed liver, Mouse non-perfused fixed cardiac muscle and Mouse perfused fixed skeletal muscle tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28007-19 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318268于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/1000. Predicted molecular weight: 52 kDa.
|
| 说明 |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Predicted molecular weight: 52 kDa. |
靶标
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功能
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. -
组织特异性
Synthesized primarily in the liver and secreted in plasma. -
疾病相关
Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis. -
序列相似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
结构域
Calcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain. -
翻译后修饰
Activated by factor XIa, which excises the activation peptide.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 14071 Mouse
- SwissProt: P16294 Mouse
- Unigene: 391283 Mouse
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别名
- Christmas Disease antibody
- Christmas factor antibody
- Coagulant factor IX antibody
see all
图片
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Western blot - Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268)All lanes : Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268) at 1/1000 dilution
Lane 1 : Mouse plasma lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lane 4 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 37,75 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: brain, skeletal muscle(PMID:2323576)
Lane4 was developed using a higher sensitivity ECL substrate.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268)](https://www.abcam.cn/ps/products/318/ab318268/Images/ab318268-2-anti-factor-ixptc-antibody-epr28007-19-immunohistochemistry-a-mouse-perfused-f.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268)Immunohistochemical analysis of paraffin-embedded (A) Mouse perfused fixed liver. (B) Mouse non-perfused fixed liver. tissue labeling Factor IX/PTC with ab318268 at 1/100 (4.91 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Positive staining in both (A) mouse perfused fixed liver and (B) mouse non-perfused fixed liver.
The section was incubated with ab318268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268)](https://www.abcam.cn/ps/products/318/ab318268/Images/ab318268-3-anti-factor-ixptc-antibody-epr28007-19-immunohistochemistry-a-mouse-perfused-f1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268)Immunohistochemical analysis of paraffin-embedded (A) Mouse perfused fixed cardiac muscle. (B) Mouse non-perfused fixed cardiac muscle. tissue labeling Factor IX/PTC with ab318268 at 1/100 (4.91 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Nearly no staining in (A) mouse perfused fixed cardiac muscle; positive staining in plasma of (B) mouse non-perfused fixed cardiac muscle.
The section was incubated with ab318268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268)](https://www.abcam.cn/ps/products/318/ab318268/Images/ab318268-4-anti-factor-ixptc-antibody-epr28007-19-immunohistochemistry-mouse-perfused-fixed.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268)Immunohistochemical analysis of paraffin-embedded Mouse perfused fixed skeletal muscle tissue labeling Factor IX/PTC with ab318268 at 1/100 (4.91 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Negative control: No staining in mouse perfused fixed skeletal muscle.
The section was incubated with ab318268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268)](https://www.abcam.cn/ps/products/318/ab318268/Images/ab318268-5-benefitsofrecombinantantibodies.jpg)
Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab318268 尚未被引用在任何文献中。