AB264397

Anti-FACL4抗体

Name: FACL4抗体 (ab264397)| Abcam中文官网
Brand: Abcam Limited
SKU: AB264397
Price: 4863 CNY
Availability: InStock

Anti-FACL4 antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal FACL4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ACSL4 aa 250-300.

查看别名

ACS4, FACL4, LACS4, ACSL4, Long-chain-fatty-acid--CoA ligase 4, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 4, LACS 4

2 Images

Supplier Data

Immunoprecipitation - Anti-FACL4 antibody (AB264397)

FACL4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264397 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab264397 at 1 μg/ml.

Lane 1 : ab264397 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgGIP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

Lysates prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-FACL4 antibody (ab264397)

Predicted band size: 79 kDa

false

Supplier Data

Western blot - Anti-FACL4 antibody (AB264397)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-FACL4 antibody (ab264397) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 79 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human ACSL4 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

O60488

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

FACL4 also known as ACSL4 is a member of the long-chain acyl-CoA synthetase (ACSL) family. This enzyme catalyzes the activation of long-chain fatty acids by forming a thioester bond with coenzyme A. FACL4 plays a critical role in lipid metabolism. The molecular weight of FACL4 is approximately 80 kDa. This protein is expressed in various tissues with a higher presence in the brain and testis reflecting its important function in lipid-related processes in these areas.

Biological function summary

FACL4 contributes significantly to lipid metabolism and is involved in the synthesis of complex lipids. It facilitates the integration of long-chain fatty acids into phospholipids and triglycerides. FACL4 often functions as part of larger lipid synthesis and modification complexes working closely with other enzymes to maintain membrane integrity and signal transduction. Its activity influences cellular responses to changes in lipid availability and metabolic demands.

Pathways

FACL4 functions within the arachidonic acid metabolism and glycerophospholipid metabolic pathways. In these pathways other proteins such as COX and LOX interact with FACL4 to regulate inflammatory responses and membrane lipid composition. The enzyme’s role in these pathways highlights its contribution to managing the balance between pro-inflammatory and anti-inflammatory metabolites which are essential for cell signaling and homeostasis.

FACL4 is implicated in several neuropsychiatric conditions including X-linked adrenoleukodystrophy and breast cancer. In the context of these diseases FACL4 interacts with proteins like ALDP in adrenoleukodystrophy. Disrupted FACL4 activity can lead to abnormal lipid accumulation or impaired lipid signaling underlying the pathophysiology of these conditions. Understanding the role of FACL4 in these disorders highlights its potential as a therapeutic target for correcting lipid metabolism dysfunction.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 21242590, PubMed : 22633490, PubMed : 24269233). Preferentially activates arachidonate and eicosapentaenoate as substrates (PubMed : 21242590). Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion (PubMed : 21242590).

See full target information ACSL4

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40244286

2025

Jacaric Acid Empowers RSL3-Induced Ferroptotic Cell Death in Two- and Three-Dimensional Breast Cancer Cell Models.

Applications

Unspecified application

Species

Unspecified reactive species

Géraldine Cuvelier,Perrine Vermonden,Pauline Debisschop,Manon Martin,Françoise Derouane,Gerhard Liebisch,Josef Ecker,Marcus Hoering,Martine Berlière,Mieke Van Bockstal,Christine Galant,François Duhoux,Larissa Mourao,Colinda Scheele,Olivier Feron,René Rezsohazy,Cyril Corbet,Yvan Larondelle

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FACL4抗体

Anti-FACL4 antibody

Immunoprecipitation - Anti-FACL4 antibody (AB264397)

Western blot - Anti-FACL4 antibody (AB264397)