重组Anti-FAAH1抗体[EPR7549] (ab128917)
![Western blot - Anti-FAAH1 antibody [EPR7549] (ab128917)](https://www.abcam.cn/ps/products/128/ab128917/Images/ab128917-142749-anti-faah1-antibody-epr7549-western-blot.jpg "Western blot - Anti-FAAH1 antibody [EPR7549] (ab128917)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7549] to FAAH1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FAAH1抗体[EPR7549]
参阅全部 FAAH1 一抗 -
描述
兔单克隆抗体[EPR7549] to FAAH1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide within Human FAAH1 aa 500-600. The exact sequence is proprietary.
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阳性对照
- Human testis, JAR and A431 cell lysates; Human brain and kidney tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 6.40 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7549 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128917于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
|
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is highly recommended. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is highly recommended. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. -
组织特异性
Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate. -
序列相似性
Belongs to the amidase family. -
细胞定位
Endomembrane system. Cytoplasm > cytoskeleton. Seems to be attached to intracellular membranes and a portion of the cytoskeletal network. - Information by UniProt
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数据库链接
- Entrez Gene: 2166 Human
- Entrez Gene: 14073 Mouse
- Omim: 602935 Human
- SwissProt: O00519 Human
- SwissProt: O08914 Mouse
- Unigene: 720143 Human
- Unigene: 256025 Mouse
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别名
- Anandamide amidohydrolase 1 antibody
- Anandamide amidohydrolase antibody
- FA2H antibody
see all
图片
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Western blot - Anti-FAAH1 antibody [EPR7549] (ab128917)All lanes : Anti-FAAH1 antibody [EPR7549] (ab128917) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : JAR cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 63 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAAH1 antibody [EPR7549] (ab128917)](https://www.abcam.cn/ps/products/128/ab128917/Images/ab128917-142748-anti-faah1-antibody-epr7549-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAAH1 antibody [EPR7549] (ab128917)ab128917, at 1/250 dilution, staining FAAH1 in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAAH1 antibody [EPR7549] (ab128917)](https://www.abcam.cn/ps/products/128/ab128917/Images/ab128917-142747-anti-faah1-antibody-epr7549-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAAH1 antibody [EPR7549] (ab128917)ab128917, at 1/250 dilution, staining FAAH1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-FAAH1 antibody [EPR7549] (ab128917)](https://www.abcam.cn/ps/products/128/ab128917/Images/ab128917-142746-anti-faah1-antibody-epr7549.jpg)
OI-RD Scanning - Anti-FAAH1 antibody [EPR7549] (ab128917) -
![Anti-FAAH1 antibody [EPR7549] (ab128917)](https://www.abcam.cn/ps/products/128/ab128917/Images/ab128917-5-benefits-of-recombinant-antibodies.png)
Anti-FAAH1 antibody [EPR7549] (ab128917)
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab128917 被引用在 6 文献中.
- Tundidor I et al. Identification of fatty acid amide hydrolase as a metastasis suppressor in breast cancer. Nat Commun 14:3130 (2023). PubMed: 37253733
- Duncan RS et al. N-acylethanolamide metabolizing enzymes are upregulated in human neural progenitor-derived neurons exposed to sub-lethal oxidative stress. Front Cell Neurosci 16:902278 (2022). PubMed: 36003139
- Li H et al. MicroRNA-1297 downregulation inhibits breast cancer cell epithelial-mesenchymal transition and proliferation in a FA2H-dependent manner. Oncol Lett 20:277 (2020). PubMed: 33014155
- Hong B et al. miR-300/FA2H affects gastric cancer cell proliferation and apoptosis. Open Med (Wars) 15:882-889 (2020). PubMed: 33344772
- Zhang K et al. CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice. J Clin Invest 129:2333-2350 (2019). PubMed: 31063987
- Aichler M et al. N-acyl Taurines and Acylcarnitines Cause an Imbalance in Insulin Synthesis and Secretion Provoking ß Cell Dysfunction in Type 2 Diabetes. Cell Metab 25:1334-1347.e4 (2017). PubMed: 28591636