Anti-FAAH1抗体[4H8] (ab54615)
Key features and details
- Mouse monoclonal [4H8] to FAAH1
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
概述
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产品名称
Anti-FAAH1抗体[4H8]
参阅全部 FAAH1 一抗 -
描述
小鼠单克隆抗体[4H8] to FAAH1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human FAAH1 aa 480-579.
Sequence:DLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGD IWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS
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常规说明
This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituents: 8% Sodium chloride, 0.6% Dibasic monohydrogen sodium phosphate, 0.2% Monobasic dihydrogen potassium phosphate, 0.2% Potassium chloride, 91% Water -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
4H8 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54615于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 24223930
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IP |
Use at an assay dependent concentration.
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IHC-P | (3) |
Use at an assay dependent concentration.
See Abreview. |
说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa. |
ICC/IF
Use at an assay dependent concentration. PubMed: 24223930 |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. See Abreview. |
靶标
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功能
Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. -
组织特异性
Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate. -
序列相似性
Belongs to the amidase family. -
细胞定位
Endomembrane system. Cytoplasm > cytoskeleton. Seems to be attached to intracellular membranes and a portion of the cytoskeletal network. - Information by UniProt
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数据库链接
- Entrez Gene: 2166 Human
- Entrez Gene: 14073 Mouse
- Entrez Gene: 29347 Rat
- Omim: 602935 Human
- SwissProt: O00519 Human
- SwissProt: O08914 Mouse
- SwissProt: P97612 Rat
- Unigene: 720143 Human
see all -
别名
- Anandamide amidohydrolase 1 antibody
- Anandamide amidohydrolase antibody
- FA2H antibody
see all
图片
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FAAH1 was immunoprecipitated using 0.5mg Mouse Liver whole tissue extract, 5µg of Mouse monoclonal to FAAH1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab54615.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 63kDa: FAAH1.This image was generated using the ascites version of the product.
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FAAH antibody (ab54615) at 1ug/lane + A-431 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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Anti-FAAH1 antibody [4H8] (ab54615) + Rat Brain
Predicted band size: 63 kDaThis image was generated using the ascites version of the product.
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Anti-FAAH1 antibody [4H8] (ab54615) + PC-12
Predicted band size: 63 kDaThis image was generated using the ascites version of the product.
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Anti-FAAH1 antibody [4H8] (ab54615) + NIH/3T3
Predicted band size: 63 kDaThis image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (31)
ab54615 被引用在 31 文献中.
- Yadav-Samudrala BJ et al. Effects of acute Δ9-tetrahydrocannabinol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice. Brain Res 1822:148638 (2024). PubMed: 37858856
- Permyakova A et al. Renal Endocannabinoid Dysregulation in Obesity-Induced Chronic Kidney Disease in Humans. Int J Mol Sci 24:N/A (2023). PubMed: 37686443
- Tripathy M et al. FAAH inhibition ameliorates breast cancer in a murine model. Oncotarget 14:910-918 (2023). PubMed: 37921652
- Pang Z et al. In situ identification of cellular drug targets in mammalian tissue. Cell 185:1793-1805.e17 (2022). PubMed: 35483372
- Xu C et al. Inhibitory Neurotransmission Is Sex-Dependently Affected by Tat Expression in Transgenic Mice and Suppressed by the Fatty Acid Amide Hydrolase Enzyme Inhibitor PF3845 via Cannabinoid Type-1 Receptor Mechanisms. Cells 11:N/A (2022). PubMed: 35269478