AB54615

Anti-FAAH1抗体[4H8]

Name: FAAH1抗体[4H8] (ab54615)| Abcam中文官网
Brand: Abcam Limited
SKU: AB54615
Price: 5309 CNY
Availability: InStock
Rating: 5 (6 reviews)

Anti-FAAH1 antibody [4H8]

(6 Reviews)

(37 Publications)

Mouse Monoclonal FAAH1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 37 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAAH aa 450 to C-terminus.

查看别名

FAAH1, FAAH, Fatty-acid amide hydrolase 1, Anandamide amidohydrolase 1, Fatty acid ester hydrolase, Oleamide hydrolase 1

5 Images

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Immunoprecipitation - Anti-FAAH1 antibody [4H8] (AB54615)

FAAH1 was immunoprecipitated using 0.5mg Mouse Liver whole tissue extract, 5μg of Mouse monoclonal to FAAH1 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70^oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab54615.
Secondary : Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band : 63kDa : FAAH1.

This image was generated using the ascites version of the product.

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Immunoprecipitation - Anti-FAAH1 antibody [4H8] (ab54615)

Predicted band size: 63 kDa

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Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

FAAH antibody (ab54615) at 1ug/lane + A-431 cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FAAH1 antibody [4H8] (ab54615)

Predicted band size: 63 kDa

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Unknown

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FAAH1 antibody [4H8] (ab54615)

All lanes:

PC-12

Predicted band size: 63 kDa

false

Unknown

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FAAH1 antibody [4H8] (ab54615)

All lanes:

Rat Brain

Predicted band size: 63 kDa

false

Unknown

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FAAH1 antibody [4H8] (ab54615)

All lanes:

NIH/3T3

Predicted band size: 63 kDa

false

不同偶联物与剂型 (2)

Carrier free

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - BSA and Azide free
Unconjugated

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - ChIP Grade

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

4H8

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, IP, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human FAAH aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00519

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "See Abreview.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "See Abreview.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "See Abreview.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.4 Constituents: 91% Water, 8% Sodium chloride, 0.6% Disodium hydrogenorthophosphate, 0.2% Potassium chloride, 0.2% Potassium phosphate monobasic

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Fatty acid amide hydrolase 1 (FAAH1) often referred to simply as FAAH is an enzyme that plays a critical role in the degradation of fatty acid amides such as anandamide in the body. This enzyme weighing approximately 63 kDa is a part of the amidase signature family and is characterized by its amidase hydrolase activity. FAAH1 is predominantly expressed in the nervous system liver and gastrointestinal tract where it hydrolyses amide bonds facilitating the termination of signaling pathways initiated by neurotransmitter-like fatty acid amides.

Biological function summary

FAAH1 is essential for maintaining homeostasis by modulating levels of bioactive lipids. It exerts control over endocannabinoid signaling by breaking down anandamide a lipid derivative functioning as a neurotransmitter. FAAH1 does not act as part of a larger protein complex. Instead it operates alone to regulate the duration and intensity of signaling rather than relying on direct interactions within a complex. The enzyme's function impacts the neurochemical milieu in tissues where it is actively expressed thereby affecting mood pain sensation and more.

Pathways

FAAH1 has a significant role in the endocannabinoid signaling pathway and fatty acid metabolism. Within these pathways it modulates signaling by hydrolysing bioactive amides like anandamide influencing overall lipid signaling. FAAH1 activities intersect with several other proteins such as cannabinoid receptors which bind the substrates that FAAH1 eventually degrades. These interactions are vital in controlling signal transduction processes that regulate physiological responses including stress and analgesia.

FAAH1 has been implicated in conditions like chronic pain and anxiety disorders. Altered FAAH1 function or expression can lead to dysregulation of endocannabinoid signaling contributing to the pathophysiology of these conditions. The enzyme's modulation of anandamide levels connects it to proteins such as CB1 and CB2 cannabinoid receptors which mediate the effects of endocannabinoids in neurological and psychiatric disorders. Research into FAAH1 inhibitors as therapeutic agents highlights its importance in potential treatments for managing pain and anxiety.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed : 17015445, PubMed : 19926788, PubMed : 9122178). Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (PubMed : 17015445, PubMed : 9122178). It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (PubMed : 21049984). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity).

See full target information FAAH

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文献 (37)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40643519

2025

Short-Term Incubation of H9c2 Cardiomyocytes with Cannabigerol Attenuates Diacylglycerol Accumulation in Lipid Overload Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Sylwia Dziemitko,Adrian Chabowski,Ewa Harasim-Symbor

Journal of neurochemistry 169:e16256 PubMed39556462

2024

Alterations of endocannabinoid signaling and microglia reactivity in the retinas of AD-like mice precede the onset of hippocampal β-amyloid plaques.

Applications

Unspecified application

Species

Unspecified reactive species

Annamaria Tisi,Lucia Scipioni,Giulia Carozza,Lucia Di Re,Giacomo Cimino,Camilla Di Meo,Sakthimala Palaniappan,Francesco Della Valle,Federico Fanti,Giacomo Giacovazzo,Dario Compagnone,Rita Maccarone,Sergio Oddi,Mauro Maccarrone

Science advances 10:eadp2622 PubMed39441940

2024

Single-cell chemoproteomics identifies metastatic activity signatures in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kavya Smitha Pillai,Olivia Laxton,Gang Li,Jing Lin,Olga Karginova,Rita Nanda,Olufunmilayo I Olopade,Savaş Tay,Raymond E Moellering

PloS one 19:e0305868 PubMed38913661

2024

Cannabinoid receptor 1 positive allosteric modulator ZCZ011 shows differential effects on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barkha J Yadav-Samudrala,Hailey Dodson,Shreya Ramineni,Elizabeth Kim,Justin L Poklis,Dai Lu,Bogna M Ignatowska-Jankowska,Aron H Lichtman,Sylvia Fitting

Pharmaceuticals (Basel, Switzerland) 17: PubMed38543105

2024

Evaluating Fatty Acid Amide Hydrolase as a Suitable Target for Sleep Promotion in a Transgenic TauP301S Mouse Model of Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Shenée C Martin,Kathryn K Joyce,Kathryn M Harper,Samuel J Harp,Todd J Cohen,Sheryl S Moy,Graham H Diering

Oncotarget 14:910-918 PubMed37921652

2023

FAAH inhibition ameliorates breast cancer in a murine model.

Applications

Unspecified application

Species

Unspecified reactive species

Mallika Tripathy,Amy Bui,Jared Henderson,Jeffrey Sun,Christian Rutan Woods,Soumya Somani,Thao Doan,Anto Sam Crosslee Louis Sam Titus,Chandra Mohan

Brain research 1822:148638 PubMed37858856

2023

Effects of acute Δ-tetrahydrocannabinol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barkha J Yadav-Samudrala,Benjamin L Gorman,Hailey Dodson,Shreya Ramineni,E Diane Wallace,Michelle R Peace,Justin L Poklis,Wei Jiang,Sylvia Fitting

International journal of molecular sciences 24: PubMed37686443

2023

Renal Endocannabinoid Dysregulation in Obesity-Induced Chronic Kidney Disease in Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Permyakova,Ariel Rothner,Sarah Knapp,Alina Nemirovski,Danny Ben-Zvi,Joseph Tam

STAR protocols 3:101778 PubMed36313539

2022

A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyuan Pang,Li Ye

Cell death and differentiation 30:168-183 PubMed36104448

2022

FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Zhu,Hao Zhang,Huawei Mao,Suqin Zhong,Yubing Huang,Sirong Chen,Kai Yan,Zhibin Zhao,Xiaohan Hao,Yue Zhang,Han Yao,Xiaowan Huang,Meimei Wang,Wenbin Zhang,Juan Li,Guangxun Meng,Xiaohua Qin,Zhiming Ye,Jiani Shen,Yang Song,Youcui Xu,Zhenyu Yang,Liansheng Wang,Yunjiao Zhang,Longping Wen

View all publications

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Anti-FAAH1抗体[4H8]