Anti-F4/80抗体[CI:A3-1] - Macrophage Marker (ab6640)
![Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.cn/ps/products/6/ab6640/Images/ab6640-266714-anti-f4-80-antibody-cia3-1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)")
Key features and details
- Rat monoclonal [CI:A3-1] to F4/80 - Macrophage Marker
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Anti-F4/80抗体[CI:A3-1] - Macrophage Marker
参阅全部 F4/80 一抗 -
描述
大鼠单克隆抗体[CI:A3-1] to F4/80 - Macrophage Marker -
宿主
Rat -
特异性
Anti-F4/80 antibody (ab6640) recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.
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经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to F4/80. Thioglycollate stimulated peritoneal macrophages from C57/BL mice
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阳性对照
- ICC/IF: Raw 264.7 cells, mouse bone marrow cells. Flow Cyt: J774 cells and peritoneal macrophages.
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常规说明
Please note we cannot guarantee IHC-P, however should you use this application for this product then please use a specific protocol which can be found in the protocols section of our datasheet here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
CI:A3-1 -
骨髓瘤
NS1 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
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- Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/10 - 1/50.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | (11) |
Use a concentration of 5 - 10 µg/ml.
We recommend ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody. |
| 说明 |
|---|
|
Flow Cyt
1/10 - 1/50. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use a concentration of 5 - 10 µg/ml. We recommend ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody. |
靶标
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功能
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
组织特异性
Expression is restricted to eosinophils. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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别名
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
ab6640 stained Raw 264.7 cells. The cells were 100% methanol fixed for 5 minutes at room temperatureand then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6640 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.cn/ps/products/6/ab6640/Images/ab6640-264399-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/10 followed by streptavidin FITC secondary at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.
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![Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.cn/ps/products/6/ab6640/Images/ab6640-12433-anti-f4-80-antibody-cia3-1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)This image is courtesy of an anonymous Abreviewab6640 staining F4/80 in mouse bone marrow cells by immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hour at 4°C. Alexa fluor® 488 goat polyclonal to rat Ig, diluted 1/200, was used as the secondary antibody. -
![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.cn/ps/products/6/ab6640/Images/ab6640-264401-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)Flow cytometry analysis of peritoneal macrophages labelling F4/80 (red) with ab6640 at a dilution of 1/10. The blue line shows J774 cells stained with Rat anti mouse isotype control.
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![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.cn/ps/products/6/ab6640/Images/ab6640-264400-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/50 followed by goat anti rat IgG FITC secondary antibody at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.
数据表及文件
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SDS download
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Datasheet download
文献 (778)
ab6640 被引用在 778 文献中.
- Caputo T et al. Anti-adipogenic signals at the onset of obesity-related inflammation in white adipose tissue. Cell Mol Life Sci 78:227-247 (2021). PubMed: 32157317
- Domínguez-Bautista JA et al. Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord. Front Cell Dev Biol 9:587096 (2021). PubMed: 33575260
- Sun B et al. Hyperoside attenuates non-alcoholic fatty liver disease through targeting Nr4A1 in macrophages. Int Immunopharmacol 94:107438 (2021). PubMed: 33611063
- Hsieh TB et al. Deletion of Calponin 2 Reduces the Formation of Postoperative Peritoneal Adhesions. J Invest Surg N/A:1-12 (2021). PubMed: 33622156
- Li Y et al. Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs. Sci Adv 7:N/A (2021). PubMed: 33627421