Anti-Ezrin (phospho Y353)抗体[EP925Y] (ab52916)
![Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916)](https://www.abcam.cn/ps/products/52/ab52916/Images/ab52916wb.jpg "Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916)")
Key features and details
- Rabbit monoclonal [EP925Y] to Ezrin (phospho Y353)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-Ezrin (phospho Y353)抗体[EP925Y]
参阅全部 Ezrin 一抗 -
描述
兔单克隆抗体[EP925Y] to Ezrin (phospho Y353) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Lysate of 293 cells treated with pervanadate.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP925Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52916于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/200000. Predicted molecular weight: 69 kDa.
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| 说明 |
|---|
|
WB
1/200000. Predicted molecular weight: 69 kDa. |
靶标
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功能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. -
组织特异性
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. -
序列相似性
Contains 1 FERM domain. -
发展阶段
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. -
翻译后修饰
Phosphorylated by tyrosine-protein kinases. -
细胞定位
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein. - Information by UniProt
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数据库链接
- Entrez Gene: 7430 Human
- Omim: 123900 Human
- SwissProt: P15311 Human
- Unigene: 487027 Human
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别名
- Villin 2 ezrin antibody
- CVIL antibody
- CVL antibody
see all
图片
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Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916)All lanes : Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916) at 1/200000 dilution
Lane 1 : 293 cell lysate untreated
Lane 2 : 293 cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab52916 被引用在 1 文献中.
- Liu P et al. Ezrin/NF-?B Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma. Med Sci Monit 24:2098-2108 (2018). WB . PubMed: 29628496