Anti-Ezrin (phospho T567)抗体(ab47293)
Key features and details
- Rabbit polyclonal to Ezrin (phospho T567)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Ezrin (phospho T567)抗体
参阅全部 Ezrin 一抗 -
描述
兔多克隆抗体to Ezrin (phospho T567) -
宿主
Rabbit -
特异性
This antibody detects endogenous levels of Ezrin only when phosphorylated at Threonine 567. This antibody will also react with Moesin at Threonine 577 and Radixin at Threonine 564. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Synthetic peptide corresponding to Human Ezrin (phospho T567).
Database link: P15311 -
阳性对照
- Extracts from A431 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was purified using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47293于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
|
| IHC-P |
Use a concentration of 1 µg/ml.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
|
IHC-P
Use a concentration of 1 µg/ml. |
靶标
-
功能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. -
组织特异性
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. -
序列相似性
Contains 1 FERM domain. -
发展阶段
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. -
翻译后修饰
Phosphorylated by tyrosine-protein kinases. -
细胞定位
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein. - Information by UniProt
-
数据库链接
- Entrez Gene: 7430 Human
- Entrez Gene: 22350 Mouse
- Entrez Gene: 54319 Rat
- Omim: 123900 Human
- SwissProt: P15311 Human
- SwissProt: P26040 Mouse
- SwissProt: P31977 Rat
- Unigene: 487027 Human
see all -
别名
- Villin 2 ezrin antibody
- CVIL antibody
- CVL antibody
see all
图片
-
Western blot - Anti-Ezrin (phospho T567) antibody (ab47293)All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)
Lane 1 : EGF treated A431 cells, no added peptide
Lane 2 : Untreated A431 cells, no added peptide
Predicted band size: 69 kDa
Observed band size: 69 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (phospho T567) antibody (ab47293)Ab47293 staining human normal placenta. Staining is localised to apical membrane and cytoplasmic compartiment.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may b -
Western blot - Anti-Ezrin (phospho T567) antibody (ab47293)All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)
Lane 1 : Jurkat cell lysate with PMA (125 ng/ml, 30 minutes)
Lane 2 : Jurkat cell lysate
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 68 kDa (possible cross reactivity)
The lower band is likely to be Moesin, which has a homologous immunogenic region.
数据表及文件
-
SDS download
-
Datasheet download
文献 (16)
ab47293 被引用在 16 文献中.
- Li F et al. Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation. Int J Biol Sci 18:1238-1253 (2022). PubMed: 35173550
- Tanaka C et al. Ezrin Modulates the Cell Surface Expression of Programmed Cell Death Ligand-1 in Human Cervical Adenocarcinoma Cells. Molecules 26:N/A (2021). PubMed: 34577118
- Broadhead MJ et al. Nanostructural Diversity of Synapses in the Mammalian Spinal Cord. Sci Rep 10:8189 (2020). PubMed: 32424125
- Chetry M et al. Effects of Galectin-1 on Biological Behavior in Cervical Cancer. J Cancer 11:1584-1595 (2020). PubMed: 32047564
- Leiphrakpam PD et al. TGFß and IGF1R signaling activates protein kinase A through differential regulation of ezrin phosphorylation in colon cancer cells. J Biol Chem 293:8242-8254 (2018). PubMed: 29599290