重组Anti-Ezrin抗体[EP924Y] (ab75840)
![Western blot - Anti-Ezrin antibody [EP924Y] (ab75840)](https://www.abcam.cn/ps/products/75/ab75840/Images/ab75840-549861-anti-ezrin-antibody-ep924y-western-blot-hek293-brain-lysates-hu-ms-rt.jpg "Western blot - Anti-Ezrin antibody [EP924Y] (ab75840)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP924Y] to Ezrin
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Ezrin抗体[EP924Y]
参阅全部 Ezrin 一抗 -
描述
兔单克隆抗体[EP924Y] to Ezrin -
宿主
Rabbit -
特异性
ab75840 detects both phosphorylated and non phosphorylated Ezrin.
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经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK 293 cells, Mouse and rat brain lysates.
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常规说明
Production method for this product has been changed from hybridoma to recombinant on 15th August 2024
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP924Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75840于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 69 kDa.
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| 说明 |
|---|
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WB
1/2000. Predicted molecular weight: 69 kDa. |
应用说明
Is unsuitable for IHC-P or IP.
靶标
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功能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. -
组织特异性
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. -
序列相似性
Contains 1 FERM domain. -
发展阶段
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. -
翻译后修饰
Phosphorylated by tyrosine-protein kinases. -
细胞定位
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein. - Information by UniProt
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数据库链接
- Entrez Gene: 7430 Human
- Entrez Gene: 22350 Mouse
- Entrez Gene: 54319 Rat
- Omim: 123900 Human
- SwissProt: P15311 Human
- SwissProt: P26040 Mouse
- SwissProt: P31977 Rat
- Unigene: 487027 Human
see all -
别名
- Villin 2 ezrin antibody
- CVIL antibody
- CVL antibody
see all
图片
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Western blot - Anti-Ezrin antibody [EP924Y] (ab75840)All lanes : Anti-Ezrin antibody [EP924Y] (ab75840) at 1/2000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 69 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time:
Lane 1: 20 seconds;
Lane 2 and Lane 3: 180 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab75840 被引用在 5 文献中.
- Liu H et al. Increased expression of IFN-? in preeclampsia impairs human trophoblast invasion via a SOCS1/JAK/STAT1 feedback loop. Exp Ther Med 21:112 (2021). PubMed: 33335575
- Li Q et al. Role of mTOR signaling in the regulation of high glucose-induced podocyte injury. Exp Ther Med 17:2495-2502 (2019). PubMed: 30906437
- Röwer C et al. Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues. Transl Oncol 11:1-10 (2017). WB ; Human . PubMed: 29132012
- Watanabe K et al. Participation of ezrin in bacterial uptake by trophoblast giant cells. Reprod Biol Endocrinol 7:95 (2009). PubMed: 19737422
- Wang G et al. MicroRNA-183 regulates Ezrin expression in lung cancer cells. FEBS Lett 582:3663-8 (2008). PubMed: 18840437