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AB168844

Anti-Exportin-5抗体

Anti-Exportin-5 antibody

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Rabbit Polyclonal Exportin-5 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human XPO5 aa 1150 to C-terminus.

查看别名

KIAA1291, RANBP21, XPO5, Exportin-5, Exp5, Ran-binding protein 21

2 Images
Western blot - Anti-Exportin-5 antibody (AB168844)
  • WB

Unknown

Western blot - Anti-Exportin-5 antibody (AB168844)

All lanes:

Western blot - Anti-Exportin-5 antibody (ab168844) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 136 kDa

true

Exposure time: 10s

Immunoprecipitation - Anti-Exportin-5 antibody (AB168844)
  • IP

Unknown

Immunoprecipitation - Anti-Exportin-5 antibody (AB168844)

Detection of Exportin-5 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168844 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Exportin-5 antibody (ab168844)

Predicted band size: 136 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human XPO5 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9HAV4

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
ab168844 was affinity purified using an epitope specific to Exportin-5 immobilized on solid support.
存储溶液
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Exportin-5 also known as Exp5 and eXportin-5 serves a mechanical role in the cellular export of specific RNA molecules from the nucleus to the cytoplasm. This protein belongs to the karyopherin family and has an approximate molecular mass of about 130 kDa. Exportin-5 primarily expresses in cell types where RNA processing and regulation are actively important though specific distribution patterns vary across different tissues.
Biological function summary

Exportin-5 functions by specifically recognizing and binding to pre-microRNA and small nuclear RNA facilitating their transport out of the nucleus. It operates as a part of a complex with RanGTP which ensures that the RNA cargo is correctly recognized and loaded for transport. This export process is essential for the subsequent RNA maturation and ultimately influences gene expression regulation.

Pathways

While Exportin-5 plays a pivotal role in the RNA transport pathway it is also intricately linked to the microRNA biogenesis pathway. Within these pathways Exportin-5 works closely with RanGTP to mediate the nuclear export of pre-microRNAs setting the stage for their maturation into functional microRNAs in the cytoplasm. It also relates to proteins involved in the RNA-induced silencing complex (RISC) assembly as mature microRNAs are integral parts of RISC.

Exportin-5 appears to be linked to tumorigenesis and certain viral infections. Abnormalities in its function may disrupt microRNA export which can lead to dysregulation of gene expression and contribute to cancer development. Furthermore associations with proteins such as Dicer and RISC-related factors highlight its role in antiviral responses where the microRNA pathway can be modulated by viral mechanisms to evade host defenses.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner.. Mediates the nuclear export of micro-RNA precursors, which form short hairpins (PubMed : 14631048, PubMed : 14681208, PubMed : 15613540). Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs (PubMed : 19965479). Binding is dependent on Ran-GTP (PubMed : 19965479).. (Microbial infection) Mediates the nuclear export of adenovirus VA1 dsRNA.
See full target information XPO5
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