重组Anti-Exportin 4/XPO4抗体[EPR4442(2)] (ab133237)
![Western blot - Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (ab133237)](https://www.abcam.cn/ps/products/133/ab133237/Images/ab133237-146640-anti-exportin-4-antibody-epr44422-western-blot.jpg "Western blot - Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (ab133237)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4442(2)] to Exportin 4/XPO4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Exportin 4/XPO4抗体[EPR4442(2)]
参阅全部 Exportin 4/XPO4 一抗 -
描述
兔单克隆抗体[EPR4442(2)] to Exportin 4/XPO4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Exportin 4/XPO4 aa 1050-1150 (C terminal).
Database link: Q0VG75 -
阳性对照
- Jurkat, HeLa, SW480, C6, Raw264.7 and PC12 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4442(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133237于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 127 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 127 kDa. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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功能
Mediates the nuclear export of proteins (cargos) with broad substrate specificity. In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. XPO4 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
序列相似性
Belongs to the exportin family. -
细胞定位
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 64328 Human
- Entrez Gene: 57258 Mouse
- Entrez Gene: 290280 Rat
- Omim: 611449 Human
- SwissProt: Q9C0E2 Human
- SwissProt: Q9ESJ0 Mouse
- Unigene: 725169 Human
- Unigene: 202747 Mouse
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别名
- Exp4 antibody
- Exportin-4 antibody
- exportin4 antibody
see all
图片
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Western blot - Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (ab133237)All lanes : Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (ab133237) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : SW480 cell lysates
Lane 4 : C6 cell lysates
Lane 5 : Raw264.7 cell lysates
Lane 6 : PC12 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 127 kDa -
![Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (ab133237)](https://www.abcam.cn/ps/products/133/ab133237/Images/ab133237-2-benefits-of-recombinant-antibodies.png)
Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (ab133237)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133237 被引用在 1 文献中.
- Balukoff NC et al. A translational program that suppresses metabolism to shield the genome. Nat Commun 11:5755 (2020). PubMed: 33188200