Anti-EXOC7抗体(ab95981)
Key features and details
- Rabbit polyclonal to EXOC7
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EXOC7抗体
参阅全部 EXOC7 一抗 -
描述
兔多克隆抗体to EXOC7 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 74 and 345 of EXOC7 (NP_001013861; UniProt ID: Q9UPT5 isoform 1).
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阳性对照
- WB: HeLa whole cell lysate; H1299, HepG2 and MOLT4 cell lysates ICC/IF: A431 cell
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95981于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 78 kDa.
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| ICC/IF |
1/100 - 1/200.
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| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 78 kDa. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion. -
序列相似性
Belongs to the EXO70 family. -
结构域
The N-terminus is involved in SEC8 and ARHQ binding.
The C-terminus is required for translocation to the plasma membrane. -
细胞定位
Cytoplasm > cytosol. Cell membrane. Translocates, as a preformed complex with SEC6 and SEC8, to the plasma membrane in response to insulin through the activation of ARHQ. - Information by UniProt
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数据库链接
- Entrez Gene: 23265 Human
- Entrez Gene: 53413 Mouse
- Omim: 608163 Human
- SwissProt: Q9UPT5 Human
- SwissProt: O35250 Mouse
- Unigene: 514496 Human
- Unigene: 22530 Mouse
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别名
- 2 5 3p antibody
- DKFZp686J04253 antibody
- EX070 antibody
see all
图片
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Western blot - Anti-EXOC7 antibody (ab95981)Anti-EXOC7 antibody (ab95981) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 78 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-EXOC7 antibody (ab95981)Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab95981 at 1/200 dilution. Lower image merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab95981 被引用在 3 文献中.
- Ulloa R et al. B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction. Front Immunol 12:801164 (2021). PubMed: 35222354
- Sáez JJ et al. The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells. J Cell Biol 218:2247-2264 (2019). PubMed: 31197029
- Arasaki K et al. Legionella remodels the plasma membrane-derived vacuole by utilizing exocyst components as tethers. J Cell Biol 217:3863-3872 (2018). PubMed: 30275106