AB124934

重组Anti-EVI1抗体[EPR5996]

Anti-EVI1 antibody [EPR5996]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal EVI1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

查看别名

EVI1, MDS1, PRDM3, MECOM, Histone-lysine N-methyltransferase MECOM, Ecotropic virus integration site 1 protein homolog, MDS1 and EVI1 complex locus protein, Myelodysplasia syndrome 1 protein, Myelodysplasia syndrome-associated protein 1, EVI-1

1 Images

Unknown

Western blot - Anti-EVI1 antibody [EPR5996] (AB124934)

All lanes:

Western blot - Anti-EVI1 antibody [EPR5996] (ab124934) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SKOV 3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit at 1/2000 dilution

Predicted band size: 118 kDa

Observed band size: 105 kDa,145 kDa,200 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-EVI1 antibody [EPR5996] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5996

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% Ethylenediaminetetraacetic acid, disodium, dihydrate

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EVI1 also known as MECOM or MECOM way is a transcription factor with a molecular mass of approximately 145 kDa. It is primarily expressed in hematopoietic stem cells and various tissues including lung and kidney. EVI1 plays a role in regulating gene expression by binding to specific DNA sequences influencing the transcription of target genes. Its expression is tightly controlled during development and aberrant expression frequently correlates with pathological conditions.

Biological function summary

EVI1 participates in the regulation of cell growth and differentiation. As part of a complex with other proteins EVI1 modulates transcriptional outcomes necessary for maintaining stem cell identity and preventing premature differentiation. It helps balance cell cycle progression and apoptosis impacting cellular responses to external stimuli. EVI1 cooperates with other transcription factors in gene regulation networks that control developmental processes.

Pathways

EVI1 interacts with several signaling pathways notably the TGF-beta and MAPK pathways. It influences TGF-beta signaling by modulating transcription factors such as SMADs which are central to this pathway. EVI1's association with MAPK signaling impacts cell proliferation and differentiation integrating multiple signaling inputs to coordinate cellular outcomes. Through these pathways EVI1 interacts with proteins like SMAD3 and ERK essential players in its regulatory circuit.

EVI1 shows a strong connection with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). In AML aberrant expression or rearrangements involving EVI1 result in blockages of normal myeloid differentiation leading to leukemic transformation. EVI1 also associates with transcription factors like GATA2 in these diseases intensifying the disruption of normal hematopoietic functions. Understanding EVI1's role offers insights into the molecular mechanisms underlying these malignancies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Isoform 1. Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis.. Isoform 7. Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation. Likely to be one of the primary histone methyltransferases along with PRDM16 that direct cytoplasmic H3K9me1 methylation.

See full target information MECOM

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:8 PubMed36609474

2023

A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yongheng Chen,Ying Ying,Maolin Wang,Canjie Ma,Min Jia,Liang Shi,Shilan Wang,Xiangyi Zheng,Wei Chen,Xing-Sheng Shu

Cell death & disease 10:166 PubMed30778048

2019

PBK, targeted by EVI1, promotes metastasis and confers cisplatin resistance through inducing autophagy in high-grade serous ovarian carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hanlin Ma,Yingwei Li,Xiangxiang Wang,Huan Wu,Gonghua Qi,Rongrong Li,Ning Yang,Min Gao,Shi Yan,Cunzhong Yuan,Beihua Kong

Nucleic acids research 47:1225-1238 PubMed30462309

2018

Direct interaction between the PRDM3 and PRDM16 tumor suppressors and the NuRD chromatin remodeling complex.

Applications

Unspecified application

Species

Unspecified reactive species

Danton Ivanochko,Levon Halabelian,Elizabeth Henderson,Pavel Savitsky,Harshika Jain,Edyta Marcon,Shili Duan,Ashley Hutchinson,Alma Seitova,Dalia Barsyte-Lovejoy,Panagis Filippakopoulos,Jack Greenblatt,Evelyne Lima-Fernandes,Cheryl H Arrowsmith

Experimental hematology 69:27-36 PubMed30352278

2018

Characterization of inv(3) cell line OCI-AML-20 with stroma-dependent CD34 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Genna M Luciani,Lihua Xie,David Dilworth,Anne Tierens,Yoni Moskovitz,Alex Murison,Magdalena M Szewczyk,Amanda Mitchell,Mathieu Lupien,Liran Shlush,John E Dick,Cheryl H Arrowsmith,Dalia Barsyte-Lovejoy,Mark D Minden

View all publications

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重组Anti-EVI1抗体[EPR5996]