Anti-ETV6 / Tel抗体(ab223705)
Key features and details
- Rabbit polyclonal to ETV6 / Tel
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ETV6 / Tel抗体
参阅全部 ETV6 / Tel 一抗 -
描述
兔多克隆抗体to ETV6 / Tel -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Cow
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免疫原
Recombinant fragment corresponding to Human ETV6/ Tel aa 50-250.
Database link: P41212 -
阳性对照
- WB: U-2 OS and CACO-2 cell lysate. IHC-P: Human breast and skin tissue. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Coimmunogen
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223705于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 53 kDa.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 53 kDa. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'. -
组织特异性
Ubiquitous. -
疾病相关
Note=A chromosomal aberration involving ETV6 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with PDGFRB. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=Chromosomal aberrations involving ETV6 are found in a form of acute myeloid leukemia (AML). Translocation t(12;22)(p13;q11) with MN1; translocation t(4;12)(q12;p13) with CHIC2.
Note=Chromosomal aberrations involving ETV6 are found in childhood acute lymphoblastic leukemia (ALL). Translocations t(12;21)(p12;q22) and t(12;21)(p13;q22) with RUNX1/AML1.
Note=A chromosomal aberration involving ETV6 is found in a form of pre-B acute myeloid leukemia. Translocation t(9;12)(p24;p13) with JAK2.
Note=A chromosomal aberration involving ETV6 is found in myelodysplastic syndrome (MDS) with basophilia. Translocation t(5;12)(q31;p13) with ACSL6.
Note=A chromosomal aberration involving ETV6 is found in acute eosinophilic leukemia (AEL). Translocation t(5;12)(q31;p13) with ACSL6.
Note=A chromosomal aberration involving ETV6 is found in myelodysplastic syndrome (MDS). Translocation t(1;12)(p36.1;p13) with MDS2.
Defects in ETV6 are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving ETV6 is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with PDGFRB on chromosome 5 creating an ETV6-PDGFRB fusion protein.
Defects in ETV6 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.
Note=A chromosomal aberration involving ETV6 is found in acute lymphoblastic leukemia. Translocation t(9;12)(p13;p13) with PAX5. -
序列相似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
翻译后修饰
Phosphorylation of Ser-257 by MAPK14 (p38) inhibits ETV6 transcriptional repression. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 504512 Cow
- Entrez Gene: 2120 Human
- Omim: 600618 Human
- SwissProt: Q0VC65 Cow
- SwissProt: P41212 Human
- Unigene: 504765 Human
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别名
- ETS related protein Tel1 antibody
- ETS translocation variant 6 antibody
- Ets variant 6 antibody
see all
图片
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Western blot - Anti-ETV6 / Tel antibody (ab223705)All lanes : Anti-ETV6 / Tel antibody (ab223705) at 0.4 µg/ml
Lane 1 : U-2-OS cell lysate
Lane 2 : CACO-2 cell lysate
Predicted band size: 53 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETV6 / Tel antibody (ab223705)Immunohistochemical analysis of human skin tissue labeling ETV6 / Tel with ab223705 at 1/200 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-ETV6 / Tel antibody (ab223705)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ETV6 / Tel (green) using ab223705 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETV6 / Tel antibody (ab223705)Immunohistochemical analysis of human breast tissue labeling ETV6 / Tel with ab223705 at 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETV6 / Tel antibody (ab223705)Immunohistochemical analysis of human liver tissue labeling ETV6 / Tel with ab223705 at 1/200 dilution. No staining is observed.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETV6 / Tel antibody (ab223705)Immunohistochemical analysis of human kidney tissue labeling ETV6 / Tel with ab223705 at 1/200 dilution. No staining is observed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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