Anti-ETS2抗体(ab272866)
Key features and details
- Rabbit polyclonal to ETS2
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ETS2抗体
参阅全部 ETS2 一抗 -
描述
兔多克隆抗体to ETS2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-P, IPmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant fragment within Human ETS2. The exact sequence is proprietary. Center region.
Database link: P15036 -
阳性对照
- WB: HeLa whole cell lysate. IHC-P: Rat forebrain tissue. ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272866于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
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| ICC/IF |
1/100 - 1/1000.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/100 - 1/500.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
ICC/IF
1/100 - 1/1000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/100 - 1/500. |
靶标
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序列相似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2114 Human
- Entrez Gene: 304063 Rat
- Omim: 164740 Human
- SwissProt: P15036 Human
- Unigene: 644231 Human
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别名
- C ets 2 protein antibody
- ETS2 antibody
- ETS2_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody (ab272866)
Paraffin-embedded rat forebrain tissue stained for ETS2 using ab272866 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody (ab272866)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ETS2 using ab272866 at 1/500 dilution (Green) in ICC/IF. Alpha-tubulin filaments (Red).
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Western blot - Anti-ETS2 antibody (ab272866)Anti-ETS2 antibody (ab272866) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody10% SDS PAGE
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Immunoprecipitation - Anti-ETS2 antibody (ab272866)IP Sample: HeLa whole cell lysate/extract
7.5% SDS-PAGE
All lanes : Anti-ETS2 antibody (ab272866) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Control with 2 µg of preimmune rabbit IgG.
Lane 3 : Immunoprecipitation of ETS2 protein by 2 µg of ab272866
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : anti-rabbit IgG HRP
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272866 尚未被引用在任何文献中。