Anti-ETS1抗体[mAbcam63302] (ab63302)
![Western blot - Anti-ETS1 antibody [mAbcam63302] (ab63302)](https://www.abcam.cn/ps/products/63/ab63302/Images/ab63302-76544-anti-ets1-antibody-mabcam63302-western-blot.jpg "Western blot - Anti-ETS1 antibody [mAbcam63302] (ab63302)")
Key features and details
- Mouse monoclonal [mAbcam63302] to ETS1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ETS1抗体[mAbcam63302]
参阅全部 ETS1 一抗 -
描述
小鼠单克隆抗体[mAbcam63302] to ETS1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Chicken, Cow, Pig
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免疫原
Recombinant fragment corresponding to Human ETS1 aa 100-250.
(Peptide available asab101223) -
阳性对照
- This antibody gave a positive signal in the following human lysates: HepG2 Nuclear; Jurkat Nuclear; HeLa Nuclear; MCF7 Whole Cell.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Buffer: 1x PBS -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
mAbcam63302 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63302于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa). |
靶标
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功能
Transcription factor. -
序列相似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
翻译后修饰
Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2113 Human
- Entrez Gene: 23871 Mouse
- Entrez Gene: 24356 Rat
- Omim: 164720 Human
- SwissProt: P14921 Human
- SwissProt: P27577 Mouse
- SwissProt: P41156 Rat
- Unigene: 369438 Human
see all -
别名
- Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
- C ets 1 protein antibody
- c-ets-1 antibody
see all
图片
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Western blot - Anti-ETS1 antibody [mAbcam63302] (ab63302)All lanes : Anti-ETS1 antibody [mAbcam63302] (ab63302) at 10 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55,60 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible isoform)
Exposure time: 2 minutes
Ab63302 is predicted to react with both isoforms of ETS1. c-ETS-1A has a predicted molecular weight of 50-kDa, whereas c-ETS-1B has a predicted molecular weight of 41-kDa. The ETS1 protein sequence contains several phosphorylation sites (Swissprot), which might explain the banding pattern observed.
数据表及文件
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Datasheet download
文献 (1)
ab63302 被引用在 1 文献中.
- Song R et al. LncRNA PAXIP1-AS1 fosters the pathogenesis of pulmonary arterial hypertension via ETS1/WIPF1/RhoA axis. J Cell Mol Med 25:7321-7334 (2021). PubMed: 34245091
