Anti-ETS1抗体(ab124282)
Key features and details
- Rabbit polyclonal to ETS1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ETS1抗体
参阅全部 ETS1 一抗 -
描述
兔多克隆抗体to ETS1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Medaka fish
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免疫原
Synthetic peptide, corresponding to a region within amino acids 175-225 of Human ETS1 (NP_005229.1).
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阳性对照
- Jurkat whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab124282 was affinity purified using an epitope specific to ETS1 immobilized on solid support . -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124282于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Transcription factor. -
序列相似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
翻译后修饰
Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2113 Human
- Entrez Gene: 23871 Mouse
- Entrez Gene: 24356 Rat
- Omim: 164720 Human
- SwissProt: P14921 Human
- SwissProt: P27577 Mouse
- SwissProt: P41156 Rat
- Unigene: 369438 Human
see all -
别名
- Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
- C ets 1 protein antibody
- c-ets-1 antibody
see all
图片
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Western blot - Anti-ETS1 antibody (ab124282)All lanes : Anti-ETS1 antibody (ab124282) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ETS1 antibody (ab124282)ab124282 at 1 µg/ml staining ETS1 in Jurkat whole cell lysate immunoprecipitated using ab124282 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG.
Predicted band size : 50 kDa. Exposure time: 30 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab124282 被引用在 8 文献中.
- Pham D et al. Batf stabilizes Th17 cell development via impaired Stat5 recruitment of Ets1-Runx1 complexes. EMBO J 42:e109803 (2023). PubMed: 36917143
- Guo M et al. Adipose-derived stem cell-derived extracellular vesicles inhibit neuroblastoma growth by regulating GABBR1 activity through LINC00622-mediated transcription factor AR. J Leukoc Biol 111:19-32 (2022). PubMed: 34448502
- Liang Y et al. Superenhancer-transcription factor regulatory network in malignant tumors. Open Med (Wars) 16:1564-1582 (2021). PubMed: 34722892
- Geng H et al. Long Noncoding RNA SNHG6 Functions as an Oncogene in Non-Small Cell Lung Cancer via Modulating ETS1 Signaling. Onco Targets Ther 13:921-930 (2020). PubMed: 32099396
- Zadora PK et al. Integrated Phosphoproteome and Transcriptome Analysis Reveals Chlamydia-Induced Epithelial-to-Mesenchymal Transition in Host Cells. Cell Rep 26:1286-1302.e8 (2019). PubMed: 30699355
- Laporte AN et al. Death by HDAC Inhibition in Synovial Sarcoma Cells. Mol Cancer Ther 16:2656-2667 (2017). PubMed: 28878027
- Laporte AN et al. HDAC and Proteasome Inhibitors Synergize to Activate Pro-Apoptotic Factors in Synovial Sarcoma. PLoS One 12:e0169407 (2017). WB ; Human . PubMed: 28056055
- Pallai R et al. Ets1 and Elk1 transcription factors regulate cancerous inhibitor of protein phosphatase 2A expression in cervical and endometrial carcinoma cells. Transcription 3:323-35 (0). PubMed: 23117818