Anti-ETFDH抗体- C-terminal (ab224663)
Key features and details
- Rabbit polyclonal to ETFDH - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ETFDH抗体- C-terminal
参阅全部 ETFDH 一抗 -
描述
兔多克隆抗体to ETFDH - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human ETFDH aa 300 to the C-terminus (C terminal).
Database link: Q16134 -
阳性对照
- WB: Mouse liver and kidney tissue lysates; HEK-293T whole cell lysate. IHC-P: Human kidney and small intestine tissues. ICC/IF: PC-3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224663于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/2000. Detects a band of approximately 68 kDa (predicted molecular weight: 63,69 kDa).
|
| IHC-P |
1/20 - 1/200.
|
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| ICC/IF |
1/50 - 1/200.
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| 说明 |
|---|
|
WB
1/500 - 1/2000. Detects a band of approximately 68 kDa (predicted molecular weight: 63,69 kDa). |
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IHC-P
1/20 - 1/200. |
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ICC/IF
1/50 - 1/200. |
靶标
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功能
Accepts electrons from ETF and reduces ubiquinone. -
疾病相关
Defects in ETFDH are the cause of glutaric aciduria type 2C (GA2C) [MIM:231680]. GA2C is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. -
序列相似性
Belongs to the ETF-QO/fixC family.
Contains 1 4Fe-4S ferredoxin-type domain. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2110 Human
- Entrez Gene: 66841 Mouse
- Omim: 231675 Human
- SwissProt: Q16134 Human
- SwissProt: Q921G7 Mouse
- Unigene: 155729 Human
- Unigene: 28336 Mouse
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别名
- Electron transfer flavoprotein ubiquinone oxidoreductase antibody
- Electron transfer flavoprotein-ubiquinone oxidoreductase antibody
- electron transferring flavoprotein dehydrogenase antibody
see all
图片
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Western blot - Anti-ETFDH antibody - C-terminal (ab224663)All lanes : Anti-ETFDH antibody - C-terminal (ab224663) at 1/500 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 63,69 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)Paraffin-embedded human kidney tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)Paraffin-embedded human small intestine tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (ab224663)PC-3 (human prostate adenocarcinoma cell line) cells stained for ETFDH (green) using ab224663 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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