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Anti-ETFB抗体(ab104944)

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Western blot - Anti-ETFB antibody (ab104944)

    Key features and details

    • Goat polyclonal to ETFB
    • Suitable for: WB, IHC-P
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-ETFB抗体
      参阅全部 ETFB 一抗

    • 描述

      山羊多克隆抗体to ETFB

    • 宿主

      Goat

    • 特异性

      ab104944 is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).

    • 经测试应用

      适用于: WB, IHC-Pmore details

    • 种属反应性

      与反应: Human
      预测可用于: Cow, Dog, Pig

    • 免疫原

      Synthetic peptide corresponding to Human ETFB aa 150-250 (internal sequence). NP_001976.1
      Database link: P38117

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • Human, Mouse and Rat Liver lysates

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab104944于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).

    1 hour primary incubation is recommended for this product.
    IHC-P
    Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).

    1 hour primary incubation is recommended for this product.
    IHC-P
    Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 功能

      The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).

    • 组织特异性

      Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas.

    • 疾病相关

      Glutaric aciduria 2B

    • 序列相似性

      Belongs to the ETF beta-subunit/FixA family.

    • 结构域

      The recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface.

    • 翻译后修饰

      Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases.

    • 细胞定位

      Mitochondrion matrix.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Beta ETF antibody
        • Beta-ETF antibody
        • Electron transfer flavoprotein beta polypeptide antibody
        • Electron transfer flavoprotein beta subunit antibody
        • Electron transfer flavoprotein subunit beta antibody
        • Electron transferring flavoprotein beta polypeptide antibody
        • etfB antibody
        • ETFB_HUMAN antibody
        • FP585 antibody
        • MADD antibody
        see all

      图片

      • Western blot - Anti-ETFB antibody (ab104944)
        Western blot - Anti-ETFB antibody (ab104944)
        Anti-ETFB antibody (ab104944) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg

        Developed using the ECL technique.

        Predicted band size: 28 kDa



        Primary incubation was 1 hour.

      文献 (2)

      发表研究结果有使用 ab104944？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab104944 被引用在 2 文献中.

      • Zhao Q  et al. Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction. Mol Med Rep 14:681-8 (2016). WB ; Rat . PubMed: 27222353
      • Stauch KL  et al. Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration. Proteomics Clin Appl 10:1205-1217 (2016). PubMed: 27568932

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