重组Anti-Estrogen Related Receptor alpha抗体[EPR46Y] (ab76228)
![Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)](https://www.abcam.cn/ps/products/76/ab76228/Images/ab76228-468755-anti-estrogen-related-receptor-alpha-antibody-epr46y-westernblot-human-mouse-rat.jpg "Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha
- Suitable for: WB, IP, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Estrogen Related Receptor alpha抗体[EPR46Y]
参阅全部 Estrogen Related Receptor alpha 一抗 -
描述
兔单克隆抗体[EPR46Y] to Estrogen Related Receptor alpha -
宿主
Rabbit -
经测试应用
适用于: WB, IP, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Estrogen Related Receptor alpha aa 1-100 (N terminal). The exact sequence is proprietary.
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阳性对照
- HeLa whole cell lysate (ab150035)
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR46Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
- Alexa Fluor® 488 Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab320364)
- Alexa Fluor® 647 Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab320365)
- PE Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab320366)
- APC Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab320367)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76228于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
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| IP |
1/20.
|
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| Flow Cyt |
1/20.
|
| 说明 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa. |
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IP
1/20. |
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Flow Cyt
1/20. |
靶标
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功能
Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
翻译后修饰
Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2101 Human
- Entrez Gene: 26379 Mouse
- Entrez Gene: 293701 Rat
- Omim: 601998 Human
- SwissProt: P11474 Human
- SwissProt: O08580 Mouse
- SwissProt: Q5QJV7 Rat
- Unigene: 110849 Human
see all -
别名
- Err 1 antibody
- ERR a antibody
- ERR alpha antibody
see all
图片
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Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)All lanes : Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa -
![Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)](https://www.abcam.cn/ps/products/76/ab76228/Images/ab76228-468754-anti-estrogen-related-receptor-alpha-antibody-epr46y-westernblot-hela-human.jpg)
Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa -
![Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)](https://www.abcam.cn/ps/products/76/ab76228/Images/ab76228-468549-anti-estrogen-related-receptor-alpha-antibody-epr46y-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)ab76228 used at 1:20 dilution (2μg) immunoprecipitating Estrogen Related Receptor alpha in Hela whole cell lysates.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg.
Lane 2 (+): HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab190685 in HeLa whole cell lysate.For western blotting, ab227648 and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.
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![Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)](https://www.abcam.cn/ps/products/76/ab76228/Images/ab76228-296557-ab76228-20171215-WB-KO-EWAR.jpg)
Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Estrogen Related Receptor alpha knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab76228 observed at 55 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab76228 was shown to specifically react with Estrogen Related Receptor alpha in wild-type HAP1 cells as signal was lost in Estrogen Related Receptor alpha knockout cells. Wild-type and Estrogen Related Receptor alpha knockout samples were subjected to SDS-PAGE. Ab76228 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)](https://www.abcam.cn/ps/products/76/ab76228/Images/ab76228-7-anti-estrogen-related-receptor-alpha-antibody-epr46y-flowcytometry-hela-human.jpg)
Flow Cytometry - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)Flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Estrogen Receptor alpha with ab76228 at 1/20 dilution (10 µg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
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![Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)](https://www.abcam.cn/ps/products/76/ab76228/Images/ab76228-5-benefits-of-recombinant-antibodies.png)
Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
数据表及文件
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SDS download
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Datasheet download
文献 (45)
ab76228 被引用在 45 文献中.
- Lau KH et al. PPARγ and C/EBPα response to acute cold stress in brown adipose tissue. iScience 26:105848 (2023). PubMed: 36624847
- Wattez JS et al. Loss of skeletal muscle estrogen-related receptors leads to severe exercise intolerance. Mol Metab 68:101670 (2023). PubMed: 36642217
- Nocito MC et al. Adrenocortical Carcinoma (ACC) Cells Rewire Their Metabolism to Overcome Curcumin Antitumoral Effects Opening a Window of Opportunity to Improve Treatment. Cancers (Basel) 15:N/A (2023). PubMed: 36831394
- Yau WW et al. Nicotinamide riboside rescues dysregulated glycolysis and fatty acid β-oxidation in a human hepatic cell model of citrin deficiency. Hum Mol Genet 32:1922-1931 (2023). PubMed: 36881658
- Su P et al. ERRα promotes glycolytic metabolism and targets the NLRP3/caspase-1/GSDMD pathway to regulate pyroptosis in endometrial cancer. J Exp Clin Cancer Res 42:274 (2023). PubMed: 37864196