Name: Estrogen Related Receptor alpha抗体[EPR46Y] (ab76228)| Abcam中文官网
Brand: Abcam Limited
SKU: AB76228
Price: 1502 CNY
Availability: InStock
Rating: 4 (1 reviews)

AB76228

KO Validated
RabMAb
Recombinant
20ul selling size
了解详情

(1 Review)

(58 Publications)

Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) is a rabbit monoclonal antibody detecting Estrogen Related Receptor alpha in Western Blot, Flow Cytometry, IP. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications
- Trusted since 2009

查看别名

ERR1, ESRL1, NR3B1, ESRRA, Steroid hormone receptor ERR1, Estrogen receptor-like 1, Estrogen-related receptor alpha, Nuclear receptor subfamily 3 group B member 1, ERR-alpha

6 Images

Flow Cytometry - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (AB76228)

Flow Cyt

Supplier Data

Flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Estrogen Receptor alpha with ab76228 at 1/20 dilution (10 μg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (AB76228)

Unknown

ab76228 used at 1 : 20 dilution (2μg) immunoprecipitating Estrogen Related Receptor alpha in Hela whole cell lysates.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg.
Lane 2 (+) : HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab190685 in HeLa whole cell lysate.

For western blotting, ab227648 and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.

All lanes:

Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

Predicted band size: 46 kDa

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Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (AB76228)

Lab

All lanes:

Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Mouse kidney lysate at 20 µg

Lane 4:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

Observed band size: 55 kDa

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Lab

All lanes:

Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

Observed band size: 55 kDa

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Lab

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : Estrogen Related Receptor alpha knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : MCF7 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab76228 observed at 55 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab76228 was shown to specifically react with Estrogen Related Receptor alpha in wild-type HAP1 cells as signal was lost in Estrogen Related Receptor alpha knockout cells. Wild-type and Estrogen Related Receptor alpha knockout samples were subjected to SDS-PAGE. ab76228 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

Predicted band size: 46 kDa

false

Lab

Western blot : Anti-ESRRA antibody [EPR46Y] (ab76228) staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab76228 was shown to bind specifically to ESRRA. A band was observed at 46 kDa in wild-type MCF7 cell lysates with no signal observed at this size in ESRRA knockout cell line. To generate this image, wild-type and ESRRA knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/2000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

Western blot - Human ESRRA knockout MCF7 cell line (ab289292)

Lane 2:

ESRRA knockout MCF7 cell lysate at 20 µg

Lane 3:

Wild-type HAP1 cell lysate at 20 µg

Lane 4:

ESRRA knockout HAP1 cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 46 kDa

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不同偶联物与剂型 (5)

Carrier free

Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Estrogen Related Receptor alpha antibody [EPR46Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Estrogen Related Receptor alpha antibody [EPR46Y]
578 PE

PE Anti-Estrogen Related Receptor alpha antibody [EPR46Y]
660 APC

APC Anti-Estrogen Related Receptor alpha antibody [EPR46Y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR46Y

亚型

IgG

不含载体蛋白

反应种属

Human, Rat, Mouse

应用

WB, IP, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP) in Human, Mouse, Rat samples.

What is the molecular weight of Estrogen Related Receptor alpha?
Anti-Estrogen Related Receptor alpha [EPR46Y] (ab76228) specifically detects a band for Estrogen Related Receptor alpha (UniProt: P11474) at a molecular weight of 55kDa.

Trusted by the scientific community
Anti-Estrogen Related Receptor alpha [EPR46Y] (ab76228) was first used in a scientific publication in 2009 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) has been confirmed by Western blot testing in ESRRA Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR46Y] also available for your convenience: ab76228, Carrier free - ab239879, Alexa Fluor® 488 - ab320364, Alexa Fluor® 647 - ab320365, PE - ab320366, APC - ab320367

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Estrogen Related Receptor Alpha (ERRα) also known as NR3B1 is an orphan nuclear receptor with a molecular mass of approximately 51 kDa. It acts as a transcription factor and influences the expression of genes involved in energy metabolism. ERRα is expressed in numerous tissues such as the liver heart kidney and skeletal muscles. It binds to DNA as a monomer and alters specific gene expression activities. This protein lacks an identified ligand which classifies it as an orphan receptor but it shares structural similarities with estrogen receptors allowing the regulation of gene transcription in several physiological contexts.

Biological function summary

Estrogen Related Receptor Alpha regulates cellular energy production processes including mitochondrial biogenesis and oxidative phosphorylation. It plays a role in the biological function of energy homeostasis and increases the expression of genes related to metabolic processes. ERRα operates as part of a transcriptional regulatory network and interacts with other nuclear receptors and coactivators to modulate its activity. It often forms complexes with coactivators such as peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) to exert its effects on mitochondrial function.

Pathways

ERRα is integral to the PGC-1α signaling pathway and the AMPK (AMP-activated protein kinase) signaling pathway. These pathways are important in regulating energy metabolism and adaptive thermogenesis. ERRα interacts with PGC-1α to enhance mitochondrial function and energy expenditure while AMPK activation affects ERRα transcriptional activity and facilitates cellular energy balance. Additionally ERRα cross-talks with other nuclear receptors like PPAR (peroxisome proliferator-activated receptor) family members which are involved in lipid metabolism and glucose homeostasis.

ERRα has implications in metabolic conditions such as diabetes and obesity. The protein links to diabetes through its role in mitochondrial dysfunction and insulin resistance. Obesity-related studies point out how ERRα affects adaptive thermogenesis and lipid metabolism contributing to energy imbalance. ERRα-PPAR interactions highlight its association in metabolic disorders influencing various aspects of metabolism and disease progression. Understanding ERRα function offers therapeutic potential for targeting metabolic diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle.

See full target information ESRRA

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文献 (58)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed41022529

2025

Antagonism of estrogen-related receptor-α inhibits mitochondrial oxidative phosphorylation and reduces M2 macrophage infiltration in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jincheng Ma,Xiaodan Mao,Yuan Ren,Xite Lin,Qibin Wu,Maotong Zhang,Jingxuan Ye,Shuxia Xu,Pingping Su,Gang Chen,Chaoyang Sun,Alexander Mustea,Pengming Sun

Nature communications 16:232 PubMed39747914

2025

The transcriptional repressor HEY2 regulates mitochondrial oxidative respiration to maintain cardiac homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Peilu She,Bangjun Gao,Dongliang Li,Chen Wu,Xuejiao Zhu,Yuan He,Fei Mo,Yao Qi,Daqing Jin,Yewei Chen,Xin Zhao,Jinzhong Lin,Hairong Hu,Jia Li,Bing Zhang,Peng Xie,Chengqi Lin,Vincent M Christoffels,Yueheng Wu,Ping Zhu,Tao P Zhong

eLife 13: PubMed39269443

2024

The bacterial quorum sensing signal 2'-aminoacetophenone rewires immune cell bioenergetics through the Ppargc1a/Esrra axis to mediate tolerance to infection.

Applications

Unspecified application

Species

Unspecified reactive species

Arijit Chakraborty,Arunava Bandyopadhaya,Vijay K Singh,Filip Kovacic,Sujin Cha,William M Oldham,A Aria Tzika,Laurence G Rahme

Cell metabolism 36:1764-1778.e9 PubMed38889724

2024

Short-term cold exposure induces persistent epigenomic memory in brown fat.

Applications

Unspecified application

Species

Unspecified reactive species

Shin-Ichi Inoue,Matthew J Emmett,Hee-Woong Lim,Mohit Midha,Hannah J Richter,Isaac J Celwyn,Rashid Mehmood,Maria Chondronikola,Samuel Klein,Amy K Hauck,Mitchell A Lazar

Cellular & molecular biology letters 29:87 PubMed38867189

2024

APOE2 protects against Aβ pathology by improving neuronal mitochondrial function through ERRα signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyuan Ning,Ying Liu,Mengyao Wan,You Zuo,Siqi Chen,Zhongshan Shi,Yongteng Xu,Honghong Li,Ho Ko,Jing Zhang,Songhua Xiao,Daji Guo,Yamei Tang

Molecular metabolism 84:101938 PubMed38631478

2024

Crosstalk interactions between transcription factors ERRα and PPARα assist PPARα-mediated gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Sofie J Desmet,Jonathan Thommis,Tineke Vanderhaeghen,Edmee M F Vandenboorn,Dorien Clarisse,Yunkun Li,Steven Timmermans,Daria Fijalkowska,Dariusz Ratman,Evelien Van Hamme,Lode De Cauwer,Bart Staels,Luc Brunsveld,Frank Peelman,Claude Libert,Jan Tavernier,Karolien De Bosscher

Molecular metabolism 83:101925 PubMed38537884

2024

Identification of a chromatin-bound ERRα interactome network in mouse liver.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Scholtes,Catherine Rosa Dufour,Emma Pleynet,Samaneh Kamyabiazar,Phillipe Hutton,Reeba Baby,Christina Guluzian,Vincent Giguère

Nature communications 14:6982 PubMed37914694

2023

Hepatocyte FBXW7-dependent activity of nutrient-sensing nuclear receptors controls systemic energy homeostasis and NASH progression in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Xia,Catherine R Dufour,Younes Medkour,Charlotte Scholtes,Yonghong Chen,Christina Guluzian,Wafa B'chir,Vincent Giguère

Journal of experimental & clinical cancer research : CR 42:274 PubMed37864196

2023

ERRα promotes glycolytic metabolism and targets the NLRP3/caspase-1/GSDMD pathway to regulate pyroptosis in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pingping Su,Xiaodan Mao,Jincheng Ma,Lixiang Huang,Lirui Yu,Shuting Tang,Mingzhi Zhuang,Zhonglei Lu,Kelvin Stefan Osafo,Yuan Ren,Xinrui Wang,Xite Lin,Leyi Huang,Xiaoli Huang,Elena Ioana Braicu,Jalid Sehouli,Pengming Sun

Molecular metabolism 78:101814 PubMed37802398

2023

ERRα fosters running endurance by driving myofiber aerobic transformation and fuel efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Xia,Charlotte Scholtes,Catherine R Dufour,Christina Guluzian,Vincent Giguère

View all publications

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