Anti-Estrogen Receptor beta (phospho S105)抗体(ab62257)
Key features and details
- Rabbit polyclonal to Estrogen Receptor beta (phospho S105)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Estrogen Receptor beta (phospho S105)抗体
参阅全部 Estrogen Receptor beta 一抗 -
描述
兔多克隆抗体to Estrogen Receptor beta (phospho S105) -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human Estrogen Receptor beta aa 50-150 (phospho S105).
Database link: Q92731 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab62257 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62257于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
|
| 说明 |
|---|
|
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. -
组织特异性
Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2100 Human
- Entrez Gene: 13983 Mouse
- Omim: 601663 Human
- SwissProt: Q92731 Human
- SwissProt: O08537 Mouse
- Unigene: 660607 Human
- Unigene: 729020 Human
- Unigene: 2561 Mouse
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别名
- ER BETA antibody
- ER-beta antibody
- Erb antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor beta (phospho S105) antibody (ab62257)ab62257 at 1/500 - 1/1000 dilution staining Estrogen Receptor beta in HuvEc cells by Immunofluorescence, in the absence or presence of the immunising peptide
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab62257 被引用在 5 文献中.
- Adelöf J et al. PA28a overexpressing female mice maintain exploratory behavior and capacity to prevent protein aggregation in hippocampus as they age. Aging Cell 20:e13336 (2021). PubMed: 33720528
- Qin L et al. Luteolin-7-O-glucoside protects dopaminergic neurons by activating estrogen-receptor-mediated signaling pathway in MPTP-induced mice. Toxicology 426:152256 (2019). PubMed: 31381935
- Adelöf J et al. PA28aß overexpression enhances learning and memory of female mice without inducing 20S proteasome activity. BMC Neurosci 19:70 (2018). PubMed: 30400847
- Siegfried JM et al. Combining the multitargeted tyrosine kinase inhibitor vandetanib with the antiestrogen fulvestrant enhances its antitumor effect in non-small cell lung cancer. J Thorac Oncol 7:485-95 (2012). PubMed: 22258476
- Hamilton-Burke W et al. Phosphorylation of Estrogen Receptor {beta} at Serine 105 Is Associated with Good Prognosis in Breast Cancer. Am J Pathol 177:1079-86 (2010). WB, IHC-P ; Human . PubMed: 20696772