Anti-Estrogen Receptor alpha (phospho S167)抗体(ab31478)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha (phospho S167)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Estrogen Receptor alpha (phospho S167)抗体
参阅全部 Estrogen Receptor alpha 一抗 -
描述
兔多克隆抗体to Estrogen Receptor alpha (phospho S167) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Estrogen Receptor alpha (phospho S167).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31478于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 68 kDa.
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| IHC-P | (1) |
1/50 - 1/100.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 68 kDa. |
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IHC-P
1/50 - 1/100. |
靶标
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功能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻译后修饰
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
细胞定位
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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数据库链接
- Entrez Gene: 2099 Human
- Omim: 133430 Human
- SwissProt: P03372 Human
- Unigene: 208124 Human
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别名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478)Immunohistochemical analysis of paraffin-embedded breast carcinoma, using ab31478 at 1/50. Left: Untreated; Right: Treated with synthesized phosphopeptide.
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Western blot - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478)All lanes : Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478) at 1/500 dilution
Lane 1 : HeLa cells treated with EGF
Lane 2 : Untreated HeLa cell lysate
Predicted band size: 68 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478)This image is courtesy of an anonymous Abreviewab31478 staining human non-small cell lung cancer tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in 10 mM sodium citrate (pH 6) prior to blocking with a commercially available blocking reagent for 20 minutes at 22°C. The primary antibody was diluted 1/200 and incubated with the sample for 24 hours at 4°C. A HRP conjugated antibody was used as the secondary.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab31478 被引用在 7 文献中.
- Muñoz JP & Calaf GM Acetylcholine, Another Factor in Breast Cancer. Biology (Basel) 12:N/A (2023). PubMed: 37998017
- Iida M et al. Compensatory role of insulin-like growth factor 1 receptor in estrogen receptor signaling pathway and possible therapeutic target for hormone therapy-resistant breast cancer. Breast Cancer 26:272-281 (2019). PubMed: 30328006
- Zhao G et al. MicroRNA-34a suppresses cell proliferation by targeting LMTK3 in human breast cancer mcf-7 cell line. DNA Cell Biol 32:699-707 (2013). WB . PubMed: 24050776
- Lucchetti C et al. The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor a in breast cancer. PLoS One 8:e55355 (2013). PubMed: 23390529
- Bobustuc GC et al. MGMT inhibition restores ERa functional sensitivity to antiestrogen therapy. Mol Med 18:913-29 (2012). PubMed: 22549111
- Skliris GP et al. A phosphorylation code for oestrogen receptor-alpha predicts clinical outcome to endocrine therapy in breast cancer. Endocr Relat Cancer 17:589-97 (2010). PubMed: 20418363
- Márquez-Garbán DC et al. Estrogen receptor signaling pathways in human non-small cell lung cancer. Steroids 72:135-43 (2007). PubMed: 17276470