Anti-Estrogen Receptor alpha (phospho S167)抗体(ab131105)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha (phospho S167)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Estrogen Receptor alpha (phospho S167)抗体
参阅全部 Estrogen Receptor alpha 一抗 -
描述
兔多克隆抗体to Estrogen Receptor alpha (phospho S167) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Estrogen Receptor alpha (phospho S167) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Sequence:LAS(p)TN
Database link: P03372 -
阳性对照
- MCF7 cell extract; MCF cells; Human breast carcinoma tissue
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab131105 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131105于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 66 kDa.
|
|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF |
1/100 - 1/200.
|
说明 |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 66 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
靶标
-
功能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻译后修饰
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
细胞定位
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
-
数据库链接
- Entrez Gene: 2099 Human
- Omim: 133430 Human
- SwissProt: P03372 Human
- Unigene: 208124 Human
-
别名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab131105)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Estrogen Receptor alpha (phospho S167) with ab131105 at 1/50 dilution. Right panel was preincubated with blocking peptide.
-
All lanes : Anti-Estrogen Receptor alpha (phospho S167) antibody (ab131105) at 1/500 dilution
Lane 1 : MCF7 cell extract with blocking peptide
Lane 2 : MCF7 cell extract
Predicted band size: 66 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab131105)Immunofluorescence analysis of methanol-fixed MCF cells labelling Estrogen Receptor alpha (phospho S167) with ab131105 at 1/100 dilution.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab131105 尚未被引用在任何文献中。