Anti-Estrogen Receptor alpha抗体(ab75635)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Estrogen Receptor alpha抗体
参阅全部 Estrogen Receptor alpha 一抗 -
描述
兔多克隆抗体to Estrogen Receptor alpha -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human, capybara -
免疫原
Synthetic peptide corresponding to Human Estrogen Receptor alpha (N terminal).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75635于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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| IP |
Use at 10 µg/mg of lysate.
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| IHC-P | (4) |
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
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IP
Use at 10 µg/mg of lysate. |
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IHC-P
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
靶标
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功能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻译后修饰
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
细胞定位
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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数据库链接
- Entrez Gene: 2099 Human
- Omim: 133430 Human
- SwissProt: P03372 Human
- Unigene: 208124 Human
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别名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
图片
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Western blot - Anti-Estrogen Receptor alpha antibody (ab75635)Anti-Estrogen Receptor alpha antibody (ab75635) at 5 µg/ml + MCF7 (Human breast adenocarcinoma cell line) cell lysate
Predicted band size: 68 kDa
Observed band size: 68 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab75635)ab75635 at 20 µg/ml dilution staining Estrogen Receptor alpha in breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab75635)This image is courtesy of an Abreview submitted by Raísa Brito.Immunohistochemical analysis of capybara testis tissue, labeling Estrogen receptor alpha with ab75635, diluted 1/1000. Heat mediated antigen retrieval with citrate buffer pH 6, blocked with BSA.
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Immunoprecipitation - Anti-Estrogen Receptor alpha antibody (ab75635)Staining of Estrogen Receptor alpha in MCF7 (Human breast adenocarcinoma cell line cell lysate immunoprecipitated using ab75635 at 10 µg/mg lysate.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (49)
ab75635 被引用在 49 文献中.
- Bouck EG et al. High risk oral contraceptive hormones do not directly enhance endothelial cell procoagulant activity in vitro. PLoS One 18:e0284333 (2023). PubMed: 37075041
- Klein-Rodewald T et al. New C3H KitN824K/WT cancer mouse model develops late-onset malignant mammary tumors with high penetrance. Sci Rep 12:19793 (2022). PubMed: 36396684
- Escandon P et al. The Role of Estriol and Estrone in Keratoconic Stromal Sex Hormone Receptors. Int J Mol Sci 23:N/A (2022). PubMed: 35055103
- Fan Q et al. miR-130a-3p promotes cell proliferation and invasion by targeting estrogen receptor a and androgen receptor in cervical cancer. Exp Ther Med 21:414 (2021). PubMed: 33747155
- Gustafsson KL et al. Arginine site 264 in murine estrogen receptor-α is dispensable for the regulation of the skeleton. Am J Physiol Endocrinol Metab 320:E160-E168 (2021). PubMed: 33225718