Anti-ESRP2抗体(ab113486)
Key features and details
- Rabbit polyclonal to ESRP2
- Suitable for: IHC-P, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ESRP2抗体
参阅全部 ESRP2 一抗 -
描述
兔多克隆抗体to ESRP2 -
宿主
Rabbit -
特异性
ab113486 is predicted to not cross-react with other ESRP family members. At least two isoforms of ESRP2 are known to exist; ab113486 will detect both.
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经测试应用
适用于: IHC-P, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide containing a 17 residue sequence from the C terminal region of Human ESRP2 (GenBank accession no. NP_079215).
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阳性对照
- WB: Human lung tissue lysate. IHC: Human lung tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113486于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 20 µg/ml.
Immunohistochemistry (Immunoflurescence detection). |
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
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IHC-P
Use a concentration of 20 µg/ml. Immunohistochemistry (Immunoflurescence detection). |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. -
组织特异性
Epithelial cell-specific. -
序列相似性
Belongs to the ESRP family.
Contains 3 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 80004 Human
- Omim: 612960 Human
- SwissProt: Q9H6T0 Human
- Unigene: 436585 Human
- Unigene: 592053 Human
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别名
- Epithelial splicing regulatory protein 2 antibody
- ESRP 2 antibody
- ESRP2 antibody
see all
图片
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Western blot - Anti-ESRP2 antibody (ab113486)Anti-ESRP2 antibody (ab113486) at 1 µg/ml + Human lung tissue lysate at 15 µg
Predicted band size: 78 kDa -
Immunohistochemistry paraffin embedded sections - Anti-ESRP2 antibody (ab113486)Immunohistochemistry (Immunofluorescence) of ESRP2 in Human lung tissue cells with ab113486 at 20 ug/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ESRP2 antibody (ab113486)Immunohistochemical analysis of Human lung tissue, staining ESRP2 with ab113486 at 5 µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab113486 被引用在 6 文献中.
- Elhasnaoui J et al. A Regulatory Axis between Epithelial Splicing Regulatory Proteins and Estrogen Receptor α Modulates the Alternative Transcriptome of Luminal Breast Cancer. Int J Mol Sci 23:N/A (2022). PubMed: 35887187
- Becker T et al. Genetic information from discordant sibling pairs points to ESRP2 as a candidate trans-acting regulator of the CF modifier gene SCNN1B. Sci Rep 10:22447 (2020). PubMed: 33384439
- Munkley J et al. Androgen-regulated transcription of ESRP2 drives alternative splicing patterns in prostate cancer. Elife 8:N/A (2019). PubMed: 31478829
- Jeong HM et al. ESRP1 is overexpressed in ovarian cancer and promotes switching from mesenchymal to epithelial phenotype in ovarian cancer cells. Oncogenesis 6:e389 (2017). PubMed: 28991261
- Deloria AJ et al. Epithelial splicing regulatory protein 1 and 2 paralogues correlate with splice signatures and favorable outcome in human colorectal cancer. Oncotarget 7:73800-73816 (2016). Human . PubMed: 27650542
- Ishii H et al. Epithelial splicing regulatory proteins 1 (ESRP1) and 2 (ESRP2) suppress cancer cell motility via different mechanisms. J Biol Chem 289:27386-99 (2014). PubMed: 25143390