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AB245062

重组Anti-ESM1抗体[EPR21097-218] - BSA and Azide free (Detector)

Anti-ESM1 antibody [EPR21097-218] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal ESM1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

查看别名

Endothelial cell-specific molecule 1, ESM-1, Esm1

1 Images
Sandwich ELISA - Anti-ESM1 antibody [EPR21097-218] - BSA and Azide free (Detector) (AB245062)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ESM1 antibody [EPR21097-218] - BSA and Azide free (Detector) (AB245062)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab236716).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21097-218

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab245062 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Endocan. The recommended pair for sandwich ELISA is:
Capture: ab244789, Mouse Endocan Capture Antibody (unconjugated)
Detector: ab245062, Mouse Endocan Detector Antibody (unconjugated)
The reference range value is 31.3 - 2000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Endothelial cell-specific molecule 1 (ESM1) also known as endocan is a proteoglycan with a mass of approximately 50 kDa. It is primarily expressed in endothelial cells of blood vessels particularly in the lungs and the kidneys. ESM1 consists of a core protein covalently attached to dermatan sulfate chains. Its expression often increases under inflammatory conditions which highlights its role in vascular biology.
Biological function summary

ESM1 acts in the regulation of cell adhesion and migration processes. It interacts with leukocytes and participates in modulating inflammatory responses. ESM1 does not form part of a multi-protein complex but its activity can influence the behavior of other cell surface molecules and receptors. This function positions ESM1 as a critical player in the dynamics of vascular endothelium.

Pathways

ESM1 modulates inflammation and angiogenesis by participating in pathways involving vascular endothelial growth factor (VEGF). It interacts with proteins such as VEGF receptor-2 impacting endothelial permeability and new blood vessel formation. These interactions situate ESM1 within the signaling networks that regulate endothelial function and vascular homeostasis.

Research associates ESM1 with conditions such as sepsis and cancer. High levels of this molecule correlate with poor prognosis in these diseases linking it functionally to VEGF and other factors involved in abnormal endothelial cell activity. Understanding ESM1's role provides insights into mechanisms of disease progression and potential therapeutic targets.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions (By similarity).
See full target information Endothelial cell-specific molecule 1
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搭配使用产品

Assay Kits

AB213776

Human ESM1 ELISA Kit (Endocan)

Sandwich ELISA - Human ESM1 ELISA Kit (Endocan) (AB213776)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB40794

Anti-FAK antibody [EP695Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)

  • 5
  • 9

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