重组Anti-ESE1抗体[EPESER1] (ab133621)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPESER1] to ESE1
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ESE1抗体[EPESER1]
参阅全部 ESE1 一抗 -
描述
兔单克隆抗体[EPESER1] to ESE1 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra)more details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to Human ESE1 aa 1 to the C-terminus.
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阳性对照
- Human uterus, fetal lung, A431, C6, Raw 264.7, PC-12, NIH3T3, and SKBR-3 cell lysates, A431 cells
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常规说明
This antibody was developed as part of a collaboration with the lab of Jim Hu at the University of Toronto.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPESER1 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133621于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 41 kDa).
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Flow Cyt (Intra) |
1/100 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 41 kDa). |
Flow Cyt (Intra)
1/100 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development. -
组织特异性
Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin. -
序列相似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
结构域
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
细胞定位
Cytoplasm. Nucleus. Localizes to the cytoplasm where it has been shown to transform MCF-12A mammary epithelial cells via a novel cytoplasmic mechanism. Also transiently expressed and localized to the nucleus where it induces apoptosis in non-transformed breast epithelial cells MCF-10A and MCF-12A via a transcription-dependent mechanism. - Information by UniProt
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数据库链接
- Entrez Gene: 1999 Human
- Entrez Gene: 13710 Mouse
- Entrez Gene: 304815 Rat
- Omim: 602191 Human
- SwissProt: P78545 Human
- SwissProt: Q3UPW2 Mouse
- SwissProt: Q4V7E1 Rat
- Unigene: 67928 Human
see all -
别名
- E74 like factor 3 antibody
- E74 like factor 3 ets domain transcription factor antibody
- E74 like factor 3 ets domain transcription factor epithelial specific antibody
see all
图片
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Overlay histogram showing A431 cells stained with ab133621 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133621, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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All lanes : Anti-ESE1 antibody [EPESER1] (ab133621) at 1/1000 dilution
Lane 1 : Human uterus cell lysate
Lane 2 : Fetal lung cell lysate
Lane 3 : A431 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH3T3 cell lysate
Lane 8 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab133621 被引用在 4 文献中.
- Lee JS et al. ELF3 Is a Target That Promotes Therapeutic Efficiency in EGFR Tyrosine Kinase Inhibitor-Resistant Non-Small Cell Lung Cancer Cells via Inhibiting PKCί. Int J Mol Sci 22:N/A (2021). PubMed: 34830169
- Yin N et al. Protein Kinase C? and Wnt/ß-Catenin Signaling: Alternative Pathways to Kras/Trp53-Driven Lung Adenocarcinoma. Cancer Cell 36:156-167.e7 (2019). PubMed: 31378680
- Enfield KSS et al. Epithelial tumor suppressor ELF3 is a lineage-specific amplified oncogene in lung adenocarcinoma. Nat Commun 10:5438 (2019). PubMed: 31780666
- Hustler A et al. Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation. Exp Cell Res N/A:N/A (2018). WB, ICC/IF ; Human . PubMed: 29842880