AB133621

重组Anti-ESE1抗体[EPESER1]

Anti-ESE1 antibody [EPESER1]

RabMAb
Recombinant
KO Validated
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(8 Publications)

Rabbit Recombinant Monoclonal ESE1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 8 publications.

查看别名

ERT, ESX, JEN, ELF3, ETS-related transcription factor Elf-3, E74-like factor 3, Epithelial-restricted with serine box, Epithelium-restricted Ets protein ESX, Epithelium-specific Ets transcription factor 1, ESE-1

3 Images

Flow Cytometry (Intracellular) - Anti-ESE1 antibody [EPESER1] (AB133621)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-ESE1 antibody [EPESER1] (AB133621)

Overlay histogram showing A431 cells stained with ab133621 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133621 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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Western blot - Anti-ESE1 antibody [EPESER1] (AB133621)

All lanes:

Western blot - Anti-ESE1 antibody [EPESER1] (ab133621) at 1/1000 dilution

Lane 1:

Human uterus cell lysate at 10 µg

Lane 2:

Fetal lung cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Lane 6:

PC12 cell lysate at 10 µg

Lane 7:

NIH3T3 cell lysate at 10 µg

Lane 8:

SKBR-3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 41 kDa

Observed band size: 47 kDa

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Lab

Western blot - Anti-ESE1 antibody [EPESER1] (AB133621)

Western blot : Rabbit Monoclonal[EPESER1] to ESE1 ab133621 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 41 kDa in Wild-type A549 cell lysates with no signal observed at this size in ELF3 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ESE1 antibody [EPESER1] (ab133621) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

ELF3 knockout A549 at 20 µg

Lane 3:

A431 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

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不同偶联物与剂型 (1)

Carrier free

Anti-ESE1 antibody [EPESER1] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPESER1

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/10000", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

This antibody was developed as part of a collaboration with the lab of Jim Hu at the University of Toronto.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ESE1 also known as ETS transcription factor ELK3 is a critical transcription factor belonging to the ETS family. This protein weighs approximately 43 kDa. ESE1 is highly expressed in epithelial tissues such as skin lung and breast. It plays an essential role in regulating gene expression by binding to specific DNA sequences and influencing transcriptional processes.

Biological function summary

ESE1 functions as a transcription activator and impacts cellular differentiation proliferation and apoptosis. It is part of a larger transcriptional complex involving additional co-activators and co-repressors. This transcription factor facilitates the control of genes tied to cell growth and development making its activity significant for normal cellular function.

Pathways

ESE1 interacts within the MAPK signaling and TGF-beta pathways influencing cellular responses to external stimuli. In the MAPK pathway ESE1 associates with proteins such as ERK1/2 to transmit signals from the cell membrane to the nucleus affecting cell fate decisions. In the TGF-beta pathway it supports regulatory mechanisms that control cell proliferation and differentiation.

Altered ESE1 expression is connected to breast cancer and psoriasis. In breast cancer ESE1 dysregulation may stimulate cancer cell growth and metastasis possibly through interaction with proteins like BRCA1 that are known for their roles in cell cycle regulation and DNA repair. In psoriasis changes in ESE1 function can lead to aberrant epidermal differentiation and inflammation further impacting the disease’s progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity).

See full target information ELF3

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 30:538 PubMed40598572

2025

DDX3X promotes endoplasmic reticulum protein reprogramming via interaction with ERN1 to amplify cerebral ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

QiaoHui Du,YuQian Liu,JingLong Guo

Cell death & disease 15:897 PubMed39695109

2024

Overexpression of ELF3 in the PTEN-deficient lung epithelium promotes lung cancer development by inhibiting ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zengzhuang Yuan,Xinyan Han,Manyu Xiao,Taoyu Zhu,Yaping Xu,Qian Tang,Chen Lian,Zijin Wang,Junming Li,Boyu Wang,Changhui Li,Xiaochen Xiang,Ruobai Jin,Yufei Liu,Xinyu Yu,Kehang Zhang,Songsong Li,Madhumita Ray,Rong Li,Artiom Gruzdev,Shiqun Shao,Fangwei Shao,Hua Wang,Wang Lian,Yong Tang,Di Chen,Ying Lei,Xuru Jin,Qinglin Li,Weiwen Long,Huaqiong Huang,Francesco J DeMayo,Jian Liu

Journal of reproductive immunology 163:104244 PubMed38555747

2024

Progesterone-mediated remodeling of the maternal-fetal interface by a PGRMC1-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wang,Leonardo M R Ferreira,Andrew Mazzanti,Huaxiao Yu,Bowen Gu,Torsten B Meissner,Qin Li,Jack L Strominger

International journal of molecular sciences 22: PubMed34830169

2021

ELF3 Is a Target That Promotes Therapeutic Efficiency in EGFR Tyrosine Kinase Inhibitor-Resistant Non-Small Cell Lung Cancer Cells via Inhibiting PKCί.

Applications

Unspecified application

Species

Unspecified reactive species

Jeon-Soo Lee,Young Eun Choi,Sunshin Kim,Ji-Youn Han,Sung-Ho Goh

Oncology letters 19:3239-3248 PubMed32256819

2020

miR-320/ELF3 axis inhibits the progression of breast cancer via the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Zhang,Jinku Zhang,Jinmei Li,Huijuan Geng,Bingjuan Zhou,Bingxin Zhang,Hong Chen

Nature communications 10:5438 PubMed31780666

2019

Epithelial tumor suppressor ELF3 is a lineage-specific amplified oncogene in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Katey S S Enfield,Erin A Marshall,Christine Anderson,Kevin W Ng,Sara Rahmati,Zhaolin Xu,Megan Fuller,Katy Milne,Daniel Lu,Rocky Shi,David A Rowbotham,Daiana D Becker-Santos,Fraser D Johnson,John C English,Calum E MacAulay,Stephen Lam,William W Lockwood,Raj Chari,Aly Karsan,Igor Jurisica,Wan L Lam

Cancer cell 36:156-167.e7 PubMed31378680

2019

Protein Kinase Cι and Wnt/β-Catenin Signaling: Alternative Pathways to Kras/Trp53-Driven Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Yin,Yi Liu,Andras Khoor,Xue Wang,E Aubrey Thompson,Michael Leitges,Verline Justilien,Capella Weems,Nicole R Murray,Alan P Fields

Experimental cell research 369:284-294 PubMed29842880

2018

Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation.

Applications

WB, ICC/IF

Species

Human, Human

Arianna Hustler,Ian Eardley,Jennifer Hinley,Joanna Pearson,Felix Wezel,Francois Radvanyi,Simon C Baker,Jennifer Southgate

View all publications

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重组Anti-ESE1抗体[EPESER1]